| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Clinical Immunology,
2 Department of Biological Psychiatry
and3 The Renal Transplantation Unit, University of Groningen, Oostersingel 59, 9713 EZ Groningen, The Netherlands
During an active human cytomegalovirus (HCMV) infection, leukocytes harbouring the HCMV lower matrix protein pp65 (ppUL83) are present in the peripheral blood and can be detected with the HCMV antigenaemia assay. In the present study, it was investigated whether the presence of pp65 in these cells was due to transcription of the virus genome or might be the result of uptake of this viral protein. Peripheral blood leukocytes of transplant recipients and AIDS patients with an active HCMV infection were investigated for the presence of HCMV immediate early (IE) antigen and pp65 using well characterized monoclonal antibodies, and for the presence of the corresponding mRNAs using non-radioactive in situ hybridization. Both mononuclear and polymorphonuclear cells were found to contain IE antigen and pp65. However, only mRNAs encoding IE antigen were found in these cells, whereas mRNAs encoding pp65 were not detected. In contrast, both IE antigen and pp65, as well as their corresponding mRNAs, were detected in the circulating late-stage HCMV-infected endothelial cells that were also present in the leukocyte fractions. These findings demonstrate that a restricted viral gene expression (transcription of IE genes) does occur in mononuclear and poly-morphonuclear leukocytes. However, the abundant presence of the early antigen pp65 without detectable presence of the corresponding mRNA in these cells strongly indicates uptake of this protein by the phagocytic leukocytes, rather than de novo synthesis.
Received 7 December 1993;
accepted 25 February 1994.
This article has been cited by other articles:
|
|
 |
|
  F. Goodrum, M. Reeves, J. Sinclair, K. High, and T. Shenk Human cytomegalovirus sequences expressed in latently infected individuals promote a latent infection in vitro Blood, August 1, 2007; 110(3): 937 - 945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Bentz, M. Jarquin-Pardo, G. Chan, M. S. Smith, C. Sinzger, and A. D. Yurochko Human Cytomegalovirus (HCMV) Infection of Endothelial Cells Promotes Naive Monocyte Extravasation and Transfer of Productive Virus To Enhance Hematogenous Dissemination of HCMV J. Virol., December 1, 2006; 80(23): 11539 - 11555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Frascaroli, S. Varani, B. Moepps, C. Sinzger, M. P. Landini, and T. Mertens Human Cytomegalovirus Subverts the Functions of Monocytes, Impairing Chemokine-Mediated Migration and Leukocyte Recruitment J. Virol., August 1, 2006; 80(15): 7578 - 7589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. McGregor, F. Liu, and M. R. Schleiss Molecular, Biological, and In Vivo Characterization of the Guinea Pig Cytomegalovirus (CMV) Homologs of the Human CMV Matrix Proteins pp71 (UL82) and pp65 (UL83) J. Virol., September 15, 2004; 78(18): 9872 - 9889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Smith, G. L. Bentz, J. S. Alexander, and A. D. Yurochko Human Cytomegalovirus Induces Monocyte Differentiation and Migration as a Strategy for Dissemination and Persistence J. Virol., May 1, 2004; 78(9): 4444 - 4453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. W. A. van der Strate, J. L. Hillebrands, S. S. Lycklama a Nijeholt, L. Beljaars, C. A. Bruggeman, M. J. A. van Luyn, J. Rozing, T. H. The, D. K. F. Meijer, G. Molema, et al. Dissemination of Rat Cytomegalovirus through Infected Granulocytes and Monocytes In Vitro and In Vivo J. Virol., October 15, 2003; 77(20): 11274 - 11278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Hansen, L. I. Strelow, D. C. Franchi, D. G. Anders, and S. W. Wong Complete Sequence and Genomic Analysis of Rhesus Cytomegalovirus J. Virol., June 15, 2003; 77(12): 6620 - 6636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Grazia Revello, F. Baldanti, E. Percivalle, A. Sarasini, L. De-Giuli, E. Genini, D. Lilleri, N. Labo, and G. Gerna In vitro selection of human cytomegalovirus variants unable to transfer virus and virus products from infected cells to polymorphonuclear leukocytes and to grow in endothelial cells J. Gen. Virol., June 1, 2001; 82(6): 1429 - 1438. [Abstract] [Full Text]
|
|
|
|
|
|
G. Gerna, E. Percivalle, F. Baldanti, S. Sozzani, P. Lanzarini, E. Genini, D. Lilleri, and M. G. Revello Human Cytomegalovirus Replicates Abortively in Polymorphonuclear Leukocytes after Transfer from Infected Endothelial Cells via Transient Microfusion Events J. Virol., June 15, 2000; 74(12): 5629 - 5638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gerna, F. Baldanti, D. Lilleri, M. Parea, E. Alessandrino, A. Pagani, F. Locatelli, J. Middeldorp, and M. G. Revello Human Cytomegalovirus Immediate-Early mRNA Detection by Nucleic Acid Sequence-Based Amplification as a New Parameter for Preemptive Therapy in Bone Marrow Transplant Recipients J. Clin. Microbiol., May 1, 2000; 38(5): 1845 - 1853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. T. Penfold, D. J. Dairaghi, G. M. Duke, N. Saederup, E. S. Mocarski, G. W. Kemble, and T. J. Schall Cytomegalovirus encodes a potent alpha chemokine PNAS, August 17, 1999; 96(17): 9839 - 9844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gerna, F. Baldanti, J. M. Middeldorp, M. Furione, M. Zavattoni, D. Lilleri, and M. G. Revello Clinical Significance of Expression of Human Cytomegalovirus pp67 Late Transcript in Heart, Lung, and Bone Marrow Transplant Recipients as Determined by Nucleic Acid Sequence-Based Amplification J. Clin. Microbiol., April 1, 1999; 37(4): 902 - 911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gallina, L. Simoncini, S. Garbelli, E. Percivalle, G. Pedrali-Noy, K. S. Lee, R. L. Erikson, B. Plachter, G. Gerna, and G. Milanesi Polo-Like Kinase 1 as a Target for Human Cytomegalovirus pp65 Lower Matrix Protein J. Virol., February 1, 1999; 73(2): 1468 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Boeckh and G. Boivin Quantitation of Cytomegalovirus: Methodologic Aspects and Clinical Applications Clin. Microbiol. Rev., July 1, 1998; 11(3): 533 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Schafer, W. Tenschert, L. Cremaschi, K. Gutensohn, and R. Laufs Utility of Major Leukocyte Subpopulations for Monitoring Secondary Cytomegalovirus Infections in Renal-Allograft Recipients by PCR J. Clin. Microbiol., April 1, 1998; 36(4): 1008 - 1014. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
