| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, U.K.
and2 Aristotelian University of Thessaloniki, Thessaloniki, Greece
Diagnosis of Crimean-Congo haemorrhagic fever (CCHF) virus infections is hampered by the problems of handling this human pathogen, which requires the highest levels of biological containment. Recombinant antigens were examined for their potential as non-hazardous diagnostic reagents. The nucleocapsid (N) gene of the Greek AP92 isolate of CCHF virus was sequenced from cloned PCR products and the open reading frame was identified by homology to the N protein of a Chinese isolate of CCHF virus. The N protein was expressed to high levels in a baculovirus expression system. Three N protein-derived peptides were expressed in Escherichia coli as fusions with glutathione S-transferase and the antigenicities of these proteins and the baculovirus-expressed protein were tested by ELISA. When tested with laboratory animal sera representing all seven serogroups of nairoviruses, the only reactive sera were those raised to CCHF virus (Greek, Nigerian and Chinese isolates) and, more weakly, Hazara virus. When tested with a panel of known positive and negative human sera, the baculovirus-expressed N protein, and the peptide derived from the central region of the N protein, proved to be the best for identifying CCHF virus-specific IgG.
Received 28 January 1994;
accepted 5 April 1994.
This article has been cited by other articles:
|
|
 |
|
  A. N. Lukashev Evidence for recombination in Crimean-Congo hemorrhagic fever virus J. Gen. Virol., August 1, 2005; 86(8): 2333 - 2338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yashina, I. Petrova, S. Seregin, O. Vyshemirskii, D. Lvov, V. Aristova, J. Kuhn, S. Morzunov, V. Gutorov, I. Kuzina, et al. Genetic variability of Crimean-Congo haemorrhagic fever virus in Russia and Central Asia J. Gen. Virol., May 1, 2003; 84(5): 1199 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Saijo, T. Qing, M. Niikura, A. Maeda, T. Ikegami, C. Prehaud, I. Kurane, and S. Morikawa Recombinant Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibodies to Crimean-Congo Hemorrhagic Fever Virus J. Clin. Microbiol., May 1, 2002; 40(5): 1587 - 1591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Saijo, T. Qing, M. Niikura, A. Maeda, T. Ikegami, K. Sakai, C. Prehaud, I. Kurane, and S. Morikawa Immunofluorescence Technique Using HeLa Cells Expressing Recombinant Nucleoprotein for Detection of Immunoglobulin G Antibodies to Crimean-Congo Hemorrhagic Fever Virus J. Clin. Microbiol., February 1, 2002; 40(2): 372 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Saeed, M. Nunes, P. F. Vasconcelos, A. P. A. Travassos Da Rosa, D. M. Watts, K. Russell, R. E. Shope, R. B. Tesh, and A. D. T. Barrett Diagnosis of Oropouche Virus Infection Using a Recombinant Nucleocapsid Protein-Based Enzyme Immunoassay J. Clin. Microbiol., July 1, 2001; 39(7): 2445 - 2452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. ter Meulen, K. Koulemou, T. Wittekindt, K. Windisch, S. Strigl, S. Conde, and H. Schmitz Detection of Lassa Virus Antinucleoprotein Immunoglobulin G (IgG) and IgM Antibodies by a Simple Recombinant Immunoblot Assay for Field Use J. Clin. Microbiol., November 1, 1998; 36(11): 3143 - 3148. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
