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Sequence of host-range determinants in the env gene of a full-length, infectious proviral clone of exogenous avian leukosis virus HPRS-103 confirms that it represents a new subgroup (designated J)

J. Bai^1,

¹ Department of Molecular Biology
and² Division of Immunology and Pathology, Institute for Animal Health, Compton, Nr Newbury, Berks RG16 0NN, UK

A genomic DNA library was constructed, in a bacteriophage vector, from line 0 chick embryo fibroblasts (CEFs) infected with HPRS-103, an exogenous avian leukosis virus (ALV; envelope subgroup J) recently isolated from meat-type chickens. The library was screened at high stringency using a full length RAV-1 (subgroup A) proviral probe. From 10⁶ plaques, two clones which hybridized strongly to the RAV-1 probe were isolated; one contained a full-length copy of the proviral genome of HPRS-103 and the other contained a copy lacking the 5'-long terminal repeat (LTR) and part of gag. The relative strength of hybridization of RAV-1 and HPRS-103 clones, to RAV-1 probes representing different parts of the proviral genome, indicated that the gag and pol genes of HPRS-103 share a high level of identity with those of RAV-1 but that the env gene and the LTRs are considerably less well conserved. Infectious virus was recovered from CEFs transfected with the full-length clone, as detected by ELISA. The recovered virus appeared to be identical to HPRS-103 by electron microscopy and by Southern blotting of proviral DNA. The recovered virus was shown to be of the same subgroup as HPRS-103 by serum neutralization and receptor interference assays. Sequence analysis of the env gene of HPRS-103 shows that it differs considerably from the env genes of other ALV subgroups, particularly in the host range determinants, consistent with the finding that HPRS-103 represents a new subgroup (designated J).

^* Author for correspondence. Fax +44 635 577263. e-mail SKINNER@BBSRC.AC.UK

Present address: Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461-1602, USA.

Received 18 August 1994; accepted 7 September 1994.

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