| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institut für Medizinische Mikrobiologie, Johannes-Gutenberg-Universität Mainz, Hochhaus am Augustusplatz, D-55101 Mainz, Germany
and2 Department of Pathology, Free University Hospital, 1081 HV Amsterdam, The Netherlands
The organization of epitopes on the minor capsid protein L2 of human papillomavirus (HPV) type 33 has been analysed using three monoclonal antibodies (MAbs) generated against a large fragment of the L2 protein (amino acids 82–259) expressed as a glutathione S-transferase fusion protein. The topology of the L2 epitopes has been investigated with respect to the structure of HPV-33 virus-like particles (VLPs). Two of the MAbs reacted with linear epitopes which were mapped to amino acids 153–160 and 163–170, respectively. These epitopes were accessible in denatured but not in native VLPs consisting of L1 and L2, suggesting an internal location. The third antibody was unable to detect denatured L2 protein but reacted with native VLPs. This is the first demonstration of an apparent conformational epitope of the HPV L2 protein. A model for the putative orientation of L2 in the papillomavirus capsid is deduced from the location of these and other antigenic sites.
* Author for correspondence. Fax +49 6131 392 359.
Received 3 May 1995;
accepted 29 June 1995.
This article has been cited by other articles:
|
|
 |
|
  M. Knappe, S. Bodevin, H.-C. Selinka, D. Spillmann, R. E. Streeck, X. S. Chen, U. Lindahl, and M. Sapp Surface-exposed Amino Acid Residues of HPV16 L1 Protein Mediating Interaction with Cell Surface Heparan Sulfate J. Biol. Chem., September 21, 2007; 282(38): 27913 - 27922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Florin, K. A. Becker, C. Lambert, T. Nowak, C. Sapp, D. Strand, R. E. Streeck, and M. Sapp Identification of a Dynein interacting domain in the papillomavirus minor capsid protein l2. J. Virol., July 1, 2006; 80(13): 6691 - 6696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kamper, P. M. Day, T. Nowak, H.-C. Selinka, L. Florin, J. Bolscher, L. Hilbig, J. T. Schiller, and M. Sapp A Membrane-Destabilizing Peptide in Capsid Protein L2 Is Required for Egress of Papillomavirus Genomes from Endosomes J. Virol., January 15, 2006; 80(2): 759 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Florin, K. A. Becker, C. Sapp, C. Lambert, H. Sirma, M. Muller, R. E. Streeck, and M. Sapp Nuclear Translocation of Papillomavirus Minor Capsid Protein L2 Requires Hsc70 J. Virol., June 1, 2004; 78(11): 5546 - 5553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Becker, L. Florin, C. Sapp, G. G. Maul, and M. Sapp Nuclear Localization but Not PML Protein Is Required for Incorporation of the Papillomavirus Minor Capsid Protein L2 into Virus-Like Particles J. Virol., February 1, 2004; 78(3): 1121 - 1128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Okun, P. M. Day, H. L. Greenstone, F. P. Booy, D. R. Lowy, J. T. Schiller, and R. B. S. Roden L1 Interaction Domains of Papillomavirus L2 Necessary for Viral Genome Encapsidation J. Virol., May 1, 2001; 75(9): 4332 - 4342. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
