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Structure and properties of a herpesvirus of turkeys recombinant in which US1, US10 and SORF3 genes have been replaced by a lacZ expression cassette

Vladimír Zelník

Institute for Animal Health, Compton Laboratory, Compton Nr Newbury, Berkshire RG16 0NN, UK

In the process of generating an insertional mutant of herpesvirus of turkeys (HVT) expressing lacZ at the protein kinase (PK) locus, we isolated a recombinant which contained an intact PK gene but the short unique regions US1, US10 and SORF3 had been deleted and replaced by the lacZ cassette. Moreover, the virus contained duplicate copies of gD, gI and gE in an opposite orientation flanking lacZ, US2 and PK which were contiguous. These results are of interest in relation to the flexibility of the short unique segment (U_s) and of the inverted repeats flanking U_s of the alpha-herpesviruses. The recombinant expressed -galactosidase and was genetically stable in vitro and in vivo. Chickens inoculated with the virus developed antibodies to HVT antigens and to -galactosidase but the replication of the recombinant in vivo was impaired in comparison to parental HVT as shown by a reduction in the proportion of infected lymphocytes.

^* Author for correspondence. Fax +44 1635 577263. e-mail ROSSN@BBSRC.ac.uk

Present address: Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 46 Bratislava, Slovak Republic.

Received 13 June 1995; accepted 5 July 1995.

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