J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 76 (1995), 2915-2922; DOI 10.1099/0022-1317-76-12-2915
© 1995 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tan, P. H. N.
Right arrow Articles by Stanley, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tan, P. H. N.
Right arrow Articles by Stanley, J.
Agricola
Right arrow Articles by Tan, P. H. N.
Right arrow Articles by Stanley, J.

Genome organization of ageratum yellow vein virus, a monopartite whitefly-transmitted geminivirus isolated from a common weed

Priscilla H. N. Tan1, Sek Man Wong1,*, Mian Wu1, Ian D. Bedford2, Keith Saunders2 and John Stanley2

1 Department of Botany, National University of Singapore, Lower Kent Ridge Road, Singapore 0511
and2 Department of Virus Research, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK

A full-length copy of a single genomic component of the whitefly-transmitted geminivirus ageratum yellow vein virus (AYVV) has been cloned from an extract of infected Ageratum conyzoides originating from Singapore. Sequence analysis shows that the genomic component encodes two virion-sense (V1 and V2) and four complementary-sense open reading frames (C1-C4), typical of DNA A of whitefly-transmitted geminiviruses from the Eastern hemisphere. A genomic component equivalent to DNA B was not detected in extracts of infected A. conyzoides. The cloned genomic component produced a systemic infection in Nicotiana benthamiana, Phaseolus vulgaris and Lycopersicon esculentum when introduced into plants by agroinoculation, and symptoms were identical to those produced by wild-type virus introduced into these hosts using viruliferous whiteflies. However, attempts to re-establish a systemic infection in A. conyzoides either by agroinoculation or by whitefly transmission of the cloned progeny were unsuccessful, suggesting that additional factors are required for infection of the natural host. The significance of A. conyzoides as a reservoir host for the economically important geminivirus diseases is discussed.

* Author for correspondence. Fax +65 777 4279. e-mail BOTWSM@LEONIS.NUS.SG

Received 8 June 1995; accepted 4 September 1995.


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
K. Saunders, I. D. Bedford, and J. Stanley
Adaptation from whitefly to leafhopper transmission of an autonomously replicating nanovirus-like DNA component associated with ageratum yellow vein disease
J. Gen. Virol., April 1, 2002; 83(4): 907 - 913.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Saunders, I. D. Bedford, R. W. Briddon, P. G. Markham, S. M. Wong, and J. Stanley
A unique virus complex causes Ageratum yellow vein disease
PNAS, June 6, 2000; 97(12): 6890 - 6895.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
L Liu, M. Pinner, J. Davies, and J Stanley
Adaptation of the geminivirus bean yellow dwarf virus to dicotyledonous hosts involves both virion-sense and complementary-sense genes
J. Gen. Virol., February 1, 1999; 80(2): 501 - 506.
[Abstract]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1995 by the Society for General Microbiology.