J Gen Virol Try Microbiology Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 76 (1995), 2941-2948; DOI 10.1099/0022-1317-76-12-2941
© 1995 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, Y.
Right arrow Articles by Miller, L. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y.
Right arrow Articles by Miller, L. K.
Agricola
Right arrow Articles by Li, Y.
Right arrow Articles by Miller, L. K.

Expression and localization of a baculovirus protein phosphatase

Yonghong Li1 and Lois K. Miller1,2,*

1 Department of Genetics
and2 Department of Entomology, The University of Georgia, Athens, GA 30602-2603, USA

We have characterized the expression of a baculoviral gene, ptp, and determined the location of its gene product, a protein tyrosine/serine phosphatase (BV-PTP), during virus infection. Using an antibody raised to a BV-PTP fusion to glutathione S-transferase, we found that ptp was expressed as a 19 kDa polypeptide at late times during virus infection. However, we also found that BV-PTP was present in the virions of both the budded and occluded forms so that a low level of BV-PTP is also present at the beginning of the infection process. Biochemical fractionation also showed that BV-PTP was primarily localized to the cytoplasm in transfected cells but that BV-PTP was present in both the cytoplasm and the nucleus of baculovirus-infected cells. Immunoelectron microscopy revealed that BV-PTP was associated with fibrillar structures which form in both the cytoplasm and the nucleus of baculovirus-infected cells.

* Author for correspondence (mail should be sent to the Department of Entomology). Fax +1 706 542 2279. e-mail Miller@bscr.uga.edu

Received 15 May 1995; accepted 9 August 1995.


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
J. Huang, B. Hao, F. Deng, X. Sun, H. Wang, and Z. Hu
Open reading frame Bm21 of Bombyx mori nucleopolyhedrovirus is not essential for virus replication in vitro, but its deletion extends the median survival time of infected larvae
J. Gen. Virol., April 1, 2008; 89(4): 922 - 930.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
J. V. d. C. Oliveira, J. L. C. Wolff, A. Garcia-Maruniak, B. M. Ribeiro, M. E. B. de Castro, M. L. de Souza, F. Moscardi, J. E. Maruniak, and P. M. d. A. Zanotto
Genome of the most widely used viral biopesticide: Anticarsia gemmatalis multiple nucleopolyhedrovirus.
J. Gen. Virol., November 1, 2006; 87(Pt 11): 3233 - 3250.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Takagi, G. S. Taylor, T. Kusakabe, H. Charbonneau, and S. Buratowski
A protein tyrosine phosphatase-like protein from baculovirus has RNA 5'-triphosphatase and diphosphatase activities
PNAS, August 18, 1998; 95(17): 9808 - 9812.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1995 by the Society for General Microbiology.