| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany
and2 Department of Immunohaematology and Bloodbank, University Hospital, Leiden, The Netherlands
CD8+ cytotoxic T cell (CTL) clones with specificity for defined minor and major histocompatibility (H) antigens were used to monitor antigen presentation in human cytomegalovirus (HCMV)-infected skin fibroblasts. At the immediate early stage of virus replication antigen presentation was intact, but was abolished during the early and late phase. Lack of CTL recognition was not selective for certain antigens but was associated with decreased steady state levels of nascent MHC class I complexes and unassembled MHC class I heavy chains, whereas free 
2-microglobulin remained abundant. HCMV also affected the stability of both immature endoglycosidase H (Endo H)-sensitive and mature Endo H-resistant MHC class I molecules, suggesting that the virus interferes with antigen presentation at more than one step during maturation of the MHC class I complex. The action of interferon-
(IFN-) and tumour necrosis factor-
(TNF-) lifted the block of MHC class I complex formation by stimulating synthesis, assembly and stability of MHC class I molecules. This resulted in restored antigen presentation provided that cells were exposed to the factors before HCMV infection. Because few MHC molecules suffice for CTL recognition these cytokines compensated for the negative viral effect on the antigen presentation function. Nevertheless, the viral interference with MHC class I complex formation was still active. The data imply that specific cytokines limit the immune evasion potential of HCMV from CD8+ T lymphocyte control.
* Author for correspondence. Fax +49 6221 563953.
Received 24 April 1995;
accepted 25 July 1995.
This article has been cited by other articles:
|
|
 |
|
  M. Trilling, V. T. K. Le, A. Zimmermann, H. Ludwig, K. Pfeffer, G. Sutter, G. L. Smith, and H. Hengel Gamma Interferon-Induced Interferon Regulatory Factor 1-Dependent Antiviral Response Inhibits Vaccinia Virus Replication in Mouse but Not Human Fibroblasts J. Virol., April 15, 2009; 83(8): 3684 - 3695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. T. K. Le, M. Trilling, M. Wilborn, H. Hengel, and A. Zimmermann Human cytomegalovirus interferes with signal transducer and activator of transcription (STAT) 2 protein stability and tyrosine phosphorylation J. Gen. Virol., October 1, 2008; 89(10): 2416 - 2426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zimmermann, M. Trilling, M. Wagner, M. Wilborn, I. Bubic, S. Jonjic, U. Koszinowski, and H. Hengel A cytomegaloviral protein reveals a dual role for STAT2 in IFN-{gamma} signaling and antiviral responses J. Exp. Med., May 16, 2005; 201(10): 1543 - 1553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hasan, A. Krmpotic, Z. Ruzsics, I. Bubic, T. Lenac, A. Halenius, A. Loewendorf, M. Messerle, H. Hengel, S. Jonjic, et al. Selective Down-Regulation of the NKG2D Ligand H60 by Mouse Cytomegalovirus m155 Glycoprotein J. Virol., March 1, 2005; 79(5): 2920 - 2930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Santibanez, S. Niewiesk, A. Heider, J. Schneider-Schaulies, G. A. M. Berbers, A. Zimmermann, A. Halenius, A. Wolbert, I. Deitemeier, A. Tischer, et al. Probing neutralizing-antibody responses against emerging measles viruses (MVs): immune selection of MV by H protein-specific antibodies? J. Gen. Virol., February 1, 2005; 86(2): 365 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Krmpotic, M. Hasan, A. Loewendorf, T. Saulig, A. Halenius, T. Lenac, B. Polic, I. Bubic, A. Kriegeskorte, E. Pernjak-Pugel, et al. NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145 J. Exp. Med., January 18, 2005; 201(2): 211 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Khan, A. Zimmermann, M. Basler, M. Groettrup, and H. Hengel A Cytomegalovirus Inhibitor of Gamma Interferon Signaling Controls Immunoproteasome Induction J. Virol., February 15, 2004; 78(4): 1831 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Atalay, A. Zimmermann, M. Wagner, E. Borst, C. Benz, M. Messerle, and H. Hengel Identification and Expression of Human Cytomegalovirus Transcription Units Coding for Two Distinct Fc{gamma} Receptor Homologs J. Virol., September 1, 2002; 76(17): 8596 - 8608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Benz, U. Reusch, W. Muranyi, W. Brune, R. Atalay, and H. Hengel Efficient downregulation of major histocompatibility complex class I molecules in human epithelial cells infected with cytomegalovirus J. Gen. Virol., September 1, 2001; 82(9): 2061 - 2070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Soderberg-Naucler, D. N. Streblow, K. N. Fish, J. Allan-Yorke, P. P. Smith, and J. A. Nelson Reactivation of Latent Human Cytomegalovirus in CD14+ Monocytes Is Differentiation Dependent J. Virol., August 15, 2001; 75(16): 7543 - 7554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Blake, T. Haigh, G. Shaka'a, D. Croom-Carter, and A. Rickinson The Importance of Exogenous Antigen in Priming the Human CD8+ T Cell Response: Lessons from the EBV Nuclear Antigen EBNA1 J. Immunol., December 15, 2000; 165(12): 7078 - 7087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ulbrecht, S. Martinozzi, M. Grzeschik, H. Hengel, J. W. Ellwart, M. Pla, and E. H. Weiss Cutting Edge: The Human Cytomegalovirus UL40 Gene Product Contains a Ligand for HLA-E and Prevents NK Cell-Mediated Lysis J. Immunol., May 15, 2000; 164(10): 5019 - 5022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Riegler, H. Hebart, H. Einsele, P. Brossart, G. Jahn, and C. Sinzger Monocyte-derived dendritic cells are permissive to the complete replicative cycle of human cytomegalovirus J. Gen. Virol., February 1, 2000; 81(2): 393 - 399. [Abstract] [Full Text]
|
|
|
|
|
|
E. Le Roy, A. Muhlethaler-Mottet, C. Davrinche, B. Mach, and J.-L. Davignon Escape of Human Cytomegalovirus from HLA-DR-Restricted CD4+ T-Cell Response Is Mediated by Repression of Gamma Interferon-Induced Class II Transactivator Expression J. Virol., August 1, 1999; 73(8): 6582 - 6589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Lehner, J. T. Karttunen, G. W. G. Wilkinson, and P. Cresswell The human cytomegalovirus US6 glycoprotein inhibits transporter associated with antigen processing-dependent peptide translocation PNAS, June 24, 1997; 94(13): 6904 - 6909. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
