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J Gen Virol 76 (1995), 3113-3118; DOI 10.1099/0022-1317-76-12-3113
© 1995 Society for General Microbiology
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The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons

M. K. Baxi1, S. Efstathiou2, G. Lawrence3, J. M. Whalley3, J. D. Slater1 and H. J. Field1,*

1 Centre for Veterinary Science, Cambridge University Veterinary School, Madingley Road, Cambridge CB3 0ES
2 Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
and3 School of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia

Neural tissues from specific pathogen-free ponies that had been experimentally infected with equine herpesvirus 1 (EHV-1) were analysed by in situ hybridization. Digoxigenin-labelled EHV-1 BamHI fragments spanning almost the entire EHV-1 genome were hybridized to RNA in tissue sections from latently infected trigeminal ganglia. The BamHI E fragment detected EHV-1 RNA antisense to gene 63 (HSV-1 homologue ICP0) in a small number of neurons. Sixteen other BamHI fragments gave negative results in 20 sections tested with each fragment. Latency associated transcripts (LATs) were localized to the neuronal nuclei. EHV-1 nucleotide sequence data in the region reveals the presence of a putative EHV-1 LAT promoter that shares similar motifs with the HSV-1 LAT promoter, including the LAT promoter-binding factor, and may have a role in EHV-1 LAT expression.

* Author for correspondence. Fax +44 1223 332998. e-mail hjf10@cam.ac.uk

Received 26 June 1995; accepted 24 July 1995.


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