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Replication-defective adenovirus type 5 as an in vitro and in vivo gene transfer vector in chickens

¹ Unité de Génétique Moléculaire, Génétique Virale, INRA, Ecole Nationale Vétérinaire d'Alfort, 94704 Maisons Alfort
and² CNEVA, Laboratoire Central de Recherches Avicole et Porcine, Unité de Pathologie Aviaire, BP 53, 22440 Ploufragan, France

The capacity of E1A gene-deleted and thus replication-defective adenovirus type 5 (Ad5) to transduce foreign genes in chicken embryo fibroblasts (CEF) as well as in chickens was investigated. The lacZ and luciferase genes were successfully transduced in CEF by replication-defective Ad5, demonstrating that these cells possess receptor(s) for binding and penetration of Ad5. A single intramuscular inoculation of Ad-gD, a replication-defective Ad5 harbouring the gD gene of pseudorabies virus, in adult and 1-day-old chickens led to the production of very high titres of specific antibodies. These gD-specific antibodies persisted for at least 56 days. These results demonstrate that replication-defective Ad5, despite its mammalian origin and the deletion of the E1A gene, is a good candidate for developing non-spreading vaccines in poultry.

^* Author for correspondence. Fax +33 143 96 71 31. e-mail MarcEloit@vet.alfort.fr

Received 20 March 1995; accepted 14 June 1995.

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