| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Veterinary Medical Research Institute
The2 Department of Microbiology, Immunology and Preventive Medicine
and The3 Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, 1802 Elwood Drive, Ames, Iowa 50011, USA
The sequences of ORFs 2 to 5 of five United States (US) porcine reproductive and respiratory syndrome virus (PRRSV) isolates with differing virulence were determined. The nucleotide and deduced amino acid sequences of these isolates were compared with those of other known PRRSV isolates. The amino acid sequence identity between seven US PRRSV isolates was 91–99% in ORF 2, 86–98% in ORF 3, 92–99% in ORF 4 and 88–97% in ORF 5. The low virulence US isolate had highest sequence variation in ORFs 2 to 4 compared to the other US isolates. A hypervariable region with antigenic potential was identified within the major envelope glycoprotein. Phylogenetic analysis of ORFs 2 to 7 indicated the existence of at least three minor genotypes within the major US genotype. The low virulence US isolate formed a branch distinct from the other US isolates. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.
* Author for correspondence. Fax +1 515 294 1401. e-mail pspaul@iastate.edu
Present address: Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Received 21 June 1995;
accepted 30 August 1995.
This article has been cited by other articles:
|
|
 |
|
  K. F. Key, D. K. Guenette, K.-J. Yoon, P. G. Halbur, T. E. Toth, and X. J. Meng Development of a Heteroduplex Mobility Assay To Identify Field Isolates of Porcine Reproductive and Respiratory Syndrome Virus with Nucleotide Sequences Closely Related to Those of Modified Live-Attenuated Vaccines J. Clin. Microbiol., June 1, 2003; 41(6): 2433 - 2439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Mateu, M. Martin, and D. Vidal Genetic diversity and phylogenetic analysis of glycoprotein 5 of European-type porcine reproductive and respiratory virus strains in Spain J. Gen. Virol., March 1, 2003; 84(3): 529 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Opriessnig, P. G. Halbur, K.-J. Yoon, R. M. Pogranichniy, K. M. Harmon, R. Evans, K. F. Key, F. J. Pallares, P. Thomas, and X. J. Meng Comparison of Molecular and Biological Characteristics of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Vaccine (Ingelvac PRRS MLV), the Parent Strain of the Vaccine (ATCC VR2332), ATCC VR2385, and Two Recent Field Isolates of PRRSV J. Virol., October 25, 2002; 76(23): 11837 - 11844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Chang, K.-J. Yoon, J. J. Zimmerman, K. M. Harmon, P. M. Dixon, C. M. T. Dvorak, and M. P. Murtaugh Evolution of Porcine Reproductive and Respiratory Syndrome Virus during Sequential Passages in Pigs J. Virol., April 16, 2002; 76(10): 4750 - 4763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Goldberg, E. C. Hahn, R. M. Weigel, and G. Scherba Genetic, geographical and temporal variation of porcine reproductive and respiratory syndrome virus in Illinois J. Gen. Virol., January 1, 2000; 81(1): 171 - 179. [Abstract] [Full Text]
|
|
|
|
|
|
E. J. Snijder, H. van Tol, K. W. Pedersen, M. J. B. Raamsman, and A. A. F. de Vries Identification of a Novel Structural Protein of Arteriviruses J. Virol., August 1, 1999; 73(8): 6335 - 6345. [Abstract] [Full Text]
|
|
|
|
|
|
R Allende, T. Lewis, Z Lu, D. Rock, G. Kutish, A Ali, A. Doster, and F. Osorio North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions J. Gen. Virol., February 1, 1999; 80(2): 307 - 315. [Abstract]
|
|
|
|
|
|
I. Morozov, T. Sirinarumitr, S. D. Sorden, P. G. Halbur, M. K. Morgan, K.-J. Yoon, and P. S. Paul Detection of a Novel Strain of Porcine Circovirus in Pigs with Postweaning Multisystemic Wasting Syndrome J. Clin. Microbiol., September 1, 1998; 36(9): 2535 - 2541. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
