HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Leonov, S. V. Articles by Norrby, E. Search for Related Content PubMed PubMed Citation Articles by Leonov, S. V. Articles by Norrby, E. Agricola Articles by Leonov, S. V. Articles by Norrby, E.

Linear antigenic and immunogenic regions of human respiratory syncytial virus N protein

¹ Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden
² Department of Virology, University of Turku, Kiinamyllynkatu 13, 20520 Turku, Finland
and The³ D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123 098 Moscow, Russia

Three linear antigenic regions on the N protein from human respiratory syncytial virus (RSV) subgroup A (strain A2) were identified by using peptides which reacted in ELISA with sera from humans with recent or previous RSV infection. The determinants were localized within three hydrophilic regions of the protein: Thr₁₁ to Gly₃₀ (N3 peptide), Ser₂₃₁ to Ala₂₅₀ (N25 peptide) and Thr₃₇₁ to Leu₃₉₁ (N39 peptide). The site represented by the N39 peptide reacted with four subgroup A-specific MAbs. There were minor variations in the amino acid epitope dependencies of each of these MAbs. Two additional antigenic regions Ser₁₃₁ to Arg₁₅₀ and Ala₁₈₁ to Leu₂₀₀, were represented by peptides that reacted with human convalescent sera, but these peptides did not differentiate between acute and convalescent sera from RSV-infected humans. Rabbit hyperimmune sera raised against selected peptides specifically precipitated different forms of the N protein from a nucleocapsid-containing homogenate derived from extracts of RSV-(subgroup A and/or B)-infected ³⁵S-labelled cells in a radioimmuneprecipitation assay (RIPA); the sera were also used to demonstrate RSV infection in cells by immunofluorescent assay (IFA). Anti-N3 peptide sera precipitated N₄₁, the full-length (M_r 41000) form of N protein, in a RIPA done on a soluble protein pool. Anti-N39 (C-terminal region) peptide sera precipitated both forms, suggesting that N₃₈ (M_r 38000) is an N-terminally truncated (probably at position Tyr₂₃ located inside the N3 peptide linear antigenic region) form of N₄₁ protein.

^* Author for correspondence. Fax +46 8 330744.

Received 5 July 1994; accepted 13 October 1994.

This article has been cited by other articles:

				J.-F. Valarcher, F. Schelcher, and H. Bourhy Evolution of Bovine Respiratory Syncytial Virus J. Virol., November 15, 2000; 74(22): 10714 - 10728. [Abstract] [Full Text]