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Servicio de Virología, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, Instituto de Salud Carlos, III, Majadahonda 28220, Madrid, Spain
To determine which human respiratory syncytial virus (HRSV) P protein serine residues are modified by cellular protein kinase(s), several mutated versions of P protein were expressed in the absence of other viral proteins. Mutations at serines 232 or 232 and 237 drastically reduced the extent of phosphorylation P protein in vivo. Serine 232 is the main site of modification and is also essential for in vitro phosphorylation by casein kinase II. Additional in vivo phosphorylation was also detected in the region containing serines 116, 117 and 119.
* Author for correspondence. Fax +34 1 639 1859.
Received 10 February 1994;
accepted 30 September 1994.
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