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1 Department of Plant Science, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
and2 Agriculture and Agri-Food Canada, 6660 NW Marine Drive, Vancouver, British Columbia V6T 1X2, Canada
The nucleotide sequence of tomato ringspot nepovirus (TomRSV) RNA1 has been determined. TomRSV RNA1 is 8214 nucleotides in length, excluding the 3' poly(A) tail, and contains a single long open reading frame (ORF) of 6591 nucleotides beginning at the first AUG codon at nucleotide position 78. This ORF accounts for 80% of the RNA1 sequence and would give rise to a polyprotein with a predicted molecular mass of 244 kDa. Amino acid sequence comparisons between portions of the TomRSV RNA1-encoded polyprotein and proteins encoded by several members of the picornavirus superfamily have provided information concerning the genomic organization and putative functions of TomRSV-encoded proteins. The putative TomRSV protease retains a conserved histidine residue present in the proteases encoded by members of the como-, poty- and poliovirus groups which is thought to be involved in dipeptide cleavage site recognition. Interestingly, this histidine residue is replaced by a leucine in the proteases of other sequenced nepoviruses. This suggests that the TomRSV protease shares dipeptide cleavage site specificity with that of como-, poty- and picornaviruses rather than the other nepoviruses.
* Author for correspondence. Fax +1 604 666 4994. e-mail ROCHON@PARGVA.AGR.CA
Present address: Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada.
Received 18 April 1994;
accepted 19 October 1994.
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