HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Katul, L. Articles by Vetten, H. J. Search for Related Content PubMed PubMed Citation Articles by Katul, L. Articles by Vetten, H. J. Agricola Articles by Katul, L. Articles by Vetten, H. J.

Sequence analysis of a faba bean necrotic yellows virus DNA component containing a putative replicase gene

Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Messeweg 11-12, 38104 Braunschweig, Germany

Faba bean necrotic yellows virus (FBNYV) has a circular ssDNA genome possibly consisting of several components of about 1 kb each. The complete nucleotide sequence of one component of FBNYV (FBNYV DNA 1) containing a putative replicase gene is presented. This component consists of 1002 nucleotides and, in the virion orientation, contains one large open reading frame (ORF1) potentially encoding a 32·3 kDa replicase with the NTP-binding motif GGEGKS. No obvious functions could be assigned to two smaller ORFs (7·4 and 9·3 kDa) occurring in the complementary orientation. Amino acid sequence comparisons of the putative replicase of FBNYV with that of other similar ssDNA viruses yielded higher homologies to subterranean clover stunt virus than to banana bunchy top and coconut foliar decay viruses. A potential stem-loop structure and a TATA box were identified within the noncoding region. Two oligonucleotides derived from FBNYV DNA 1 were used for direct sequencing of the virion ssDNA to determine its virion polarity and for amplifying part of this component by immunocapture PCR in extracts from FBNYV-infected plants.

^* Author for correspondence. Fax +49 531 299 3006. e-mail UN381EM@GENIUS.EMBNET.DKFZ-HEIDELBERG.DE

Received 8 August 1994; accepted 4 October 1994.

This article has been cited by other articles:

				T. Timchenko, L. Katul, M. Aronson, J. C. Vega-Arreguin, B. C. Ramirez, H. J. Vetten, and B. Gronenborn Infectivity of nanovirus DNAs: induction of disease by cloned genome components of Faba bean necrotic yellows virus J. Gen. Virol., June 1, 2006; 87(6): 1735 - 1743. [Abstract] [Full Text] [PDF]

				C. L. Horser, R. M. Harding, and J. L. Dale Banana bunchy top nanovirus DNA-1 encodes the 'master' replication initiation protein J. Gen. Virol., February 1, 2001; 82(2): 459 - 464. [Abstract] [Full Text]

				M. N. Aronson, A. D. Meyer, J. Györgyey, L. Katul, H. J. Vetten, B. Gronenborn, and T. Timchenko Clink, a Nanovirus-Encoded Protein, Binds both pRB and SKP1 J. Virol., April 1, 2000; 74(7): 2967 - 2972. [Abstract] [Full Text]

				T. Timchenko, F. de Kouchkovsky, L. Katul, C. David, H. J. Vetten, and B. Gronenborn A Single Rep Protein Initiates Replication of Multiple Genome Components of Faba Bean Necrotic Yellows Virus, a Single-Stranded DNA Virus of Plants J. Virol., December 1, 1999; 73(12): 10173 - 10182. [Abstract] [Full Text]