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In situ localization of the non-structural protein P25 encoded by beet necrotic yellow vein virus RNA 3

A. M. Haeberlé^1,

¹ INRA, Station Grandes Cultures, Laboratoire de Pathologie Végétale, 68021 Colmar Cedex
and² Institut de Biologie Moléculaire des Plantes et Université Louis Pasteur de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France

The in situ localization of the non-structural protein P25 encoded by beet necrotic yellow vein virus (BNYVV) RNA 3 and of the BNYVV coat protein (CP) was studied by immunoelectron microscopy in infected leaf and root cells of Chenopodium murale and C. quinoa. The CP was detected in the cytoplasm of all cell types except xylem, sieve elements, and companion cells. P25 was detected in the cytoplasm and nuclei of the same cell types. The intensity of CP labelling varied depending upon the stage of infection of the cell, whereas the P25 labelling intensity was similar in newly infected cells and in cells at later stages of infection. These results suggest that P25 may be synthesized at an earlier stage of infection than CP. Its presence in the nuclei of newly infected cells may be related to the reported effect of P25 on leaf symptom development.

^* Author for correspondence. Fax +33 88 61 44 42. e-mail L409@MEDOC.U-STRASBG.FR

Present address: Biologische Bundesanstalt für Land- und Forst-wirtschaft, Institute für Biochemie und Planzenvirologie, Messeweg 11, D-W-3300 Braunschweig, Germany.

Received 25 August 1994; accepted 27 October 1994.

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