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Repair of phage DNA damaged by near ultraviolet light plus 8-methoxypsoralen

Department of Life Sciences, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK

Treatment of phage with 8-methoxypsoralen plus near ultraviolet light (PUVA) and its subsequent infection and growth on various mutant and non-mutant hosts were investigated. A number of Escherichia coli DNA repair-deficient mutants, particularly those deficient in genes producing proteins known to participate in interstrand crosslink repair, were used as hosts to assess the roles of these gene products in the activation of phage affected by PUVA. Results show that puvA, uvrA, uvrD, recA, recO, sulA and recN of E. coli are involved in the repair process. Based on the data presented it is proposed that phage DNA is repaired, following PUVA damage, using the recombinational repair process. This may be in agreement with the recombinational model of the repair of E. coli DNA.

^* Author for correspondence. Fax +44 1602 486636. e-mail LIF3AHMADSI@NTU

Received 16 August 1994; accepted 7 November 1994.