| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Laboratory of Viral Pathogenesis, Biomedical Primate Research Centre, PO Box 5815, 2280 HV Rijswijk
2 Human Retrovirus Laboratory, Academic Medical Centre, Amsterdam, the Netherlands
3 Laboratory of Microbiology, Institute of Tropical Medicine, Antwerpen
and4 SmithKline Beecham Biologicals, Rixensart, Belgium
Many reports indicate that a long-term asymptomatic state following human immunodeficiency virus type 1 (HIV-1) infection is associated with a low amount of circulating virus. To evaluate the possible effect of stabilizing a low virus load by non-sterilizing preexposure vaccination, a quantitative virus isolation method was developed and evaluated in four chronically infected chimpanzees infected with a variety of HIV-1 related isolates. This assay was then used to monitor a group of chimpanzees (n = 6) challenged with HIV-1 following vaccination with gp120 or gp160. Data indicated that of the three vaccinated animals which became infected after challenge, the animal with the lowest neutralizing titre at the time of challenge acquired a virus load similar to the control animals, whereas the two other chimpanzees had reduced numbers of virus producing cells in their peripheral circulation. One animal became virus isolation negative, developed an indeterminant PCR signal on lymph node DNA and subsequently became negative for HIV-1 DNA as determined by PCR on PBMC (peripheral blood mononuclear cells) and bone marrow DNA. Recently, the second animal has also become PCR negative. To confirm observations from quantitative virus isolations, quantification of HIV-1 DNA in PBMC and virus RNA in serum was performed by PCR on serially diluted samples at two different time points. Comparison of virus load as determined by these three methods confirmed that there was an effect of vaccination in reducing virus load and demonstrated a correlation between decreased numbers of virus producing cells, HIV-1 DNA containing cells and virus RNA molecules in serum.
* Author for correspondence. Fax +31 15 84 39 86. e-mail heeney@mbl.tno.nl
Received 7 October 1994;
accepted 16 December 1994.
This article has been cited by other articles:
|
|
 |
|
  P. t. Haaft, E. J. Verschoor, B. Verstrepen, H. Niphuis, R. Dubbes, W. Koornstra, W. Bogers, B. Rosenwirth, and J. L. Heeney Readily acquired secondary infections of human and simian immunodeficiency viruses following single intravenous exposure in non-human primates J. Gen. Virol., December 1, 2004; 85(12): 3735 - 3745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Mooij, I. G. Nieuwenhuis, C. J. Knoop, R. W. Doms, W. M. J. M. Bogers, P. J. F. ten Haaft, H. Niphuis, W. Koornstra, K. Bieler, J. Kostler, et al. Qualitative T-Helper Responses to Multiple Viral Antigens Correlate with Vaccine-Induced Immunity to Simian/Human Immunodeficiency Virus Infection J. Virol., April 1, 2004; 78(7): 3333 - 3342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Santiago, M. Lukasik, S. Kamenya, Y. Li, F. Bibollet-Ruche, E. Bailes, M. N. Muller, M. Emery, D. A. Goldenberg, J. S. Lwanga, et al. Foci of Endemic Simian Immunodeficiency Virus Infection in Wild-Living Eastern Chimpanzees (Pan troglodytes schweinfurthii) J. Virol., July 1, 2003; 77(13): 7545 - 7562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Balla-Jhagjhoorsingh, G. Koopman, P. Mooij, T. G. M. Haaksma, V. J. P. Teeuwsen, R. E. Bontrop, and J. L. Heeney Conserved CTL Epitopes Shared Between HIV-Infected Human Long-Term Survivors and Chimpanzees J. Immunol., February 15, 1999; 162(4): 2308 - 2314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ten Haaft, B. Verstrepen, K. Uberla, B. Rosenwirth, and J. Heeney A Pathogenic Threshold of Virus Load Defined in Simian Immunodeficiency Virus- or Simian-Human Immunodeficiency Virus-Infected Macaques J. Virol., December 1, 1998; 72(12): 10281 - 10285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Heeney, V. J. P. Teeuwsen, M. van Gils, W. M. J. M. Bogers, C. D. G. Morghen, A. Radaelli, S. Barnett, B. Morein, L. Akerblom, Y. Wang, et al. beta -Chemokines and neutralizing antibody titers correlate with sterilizing immunity generated in HIV-1 vaccinated macaques PNAS, September 1, 1998; 95(18): 10803 - 10808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. I. Connor, B. T. M. Korber, B. S. Graham, B. H. Hahn, D. D. Ho, B. D. Walker, A. U. Neumann, S. H. Vermund, J. Mestecky, S. Jackson, et al. Immunological and Virological Analyses of Persons Infected by Human Immunodeficiency Virus Type 1 while Participating in Trials of Recombinant gp120 Subunit Vaccines J. Virol., February 1, 1998; 72(2): 1552 - 1576. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
