HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bach, C. Articles by Scholtissek, C. Search for Related Content PubMed PubMed Citation Articles by Bach, C. Articles by Scholtissek, C. Agricola Articles by Bach, C. Articles by Scholtissek, C.

Effect of methyltransferase inhibitors on the regulation of baculovirus protein synthesis

¹ Institut für Virologie, Justus-Liebig-Universität Giessen, Frankfurter Strasse 107, D-35392 Giessen
and² Institut für Virologie, Philipps-Universität, Robert Koch-Strasse 17, D-35037 Marburg, Germany

In the presence of the methyltransferase inhibitor 3-deazaadenosine (3DA-Ado) the production of infectious Autographa californica nuclear polyhedrosis virus (AcMNPV) in tissue culture was only slightly affected, while the synthesis of very late proteins (polyhedrin and p10) was abolished. The synthesis of the influenza virus proteins NS1 and HA, expressed under the polyhedrin promoter, was also abolished by 3DA-Ado. Furthermore, 3DA-Ado interfered with the shut-off of early and late AcMNPV proteins. Most of these results were also obtained with 5-azadeoxycytidine (5A-dCyt). In cells in which NS1 was produced abundantly, at least one specific AcMNPV protein was not synthesized. However, if the production of NS1 was inhibited by 3DA-Ado, or if HA was synthesized instead, this AcMNPV protein showed up normally.

^* Author for correspondence. Fax +49 641 23960.

Received 26 August 1994; accepted 6 December 1994.