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Differences in the target specificity of the transactivating factors MHBs^t and HBx of hepatitis B virus

Max-Planck-Institut für Biochemie, Department of Virus Research, Am Klopferspitz 18a, D-82152 Martinsried, Germany

Transient transfections of tissue culture cells with plasmids encoding the transactivating factors MHBs^t and HBx of hepatitis B virus result in transcriptional stimulation of multiple target genes. Our experiments show that the NF-B-binding enhancer element of simian virus 40 (SV40) and the AP-1-binding enhancer element of the human metallothionein IIA gene mediate the transactivating function of MHBs^t and HBx. In contrast, the elements GT(IIC+I) and Sph(II+I) of the SV40 enhancer, that, as a common feature, require binding of transcription factor TEF-1 for activity, efficiently mediate transactivation only by HBx but not by MHBs^t. This finding suggests that at least one regulatory pathway exists that can only be activated by HBx but not by MHBs^t.

^* Author for correspondence. Present address: German Heart Center Munich, Department of Experimental Cardiology, Lothstrasse 11, D-80335 Munich, Germany. Fax +49 891209549.

Received 15 September 1994; accepted 10 November 1994.