J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 76 (1995), 1047-1050; DOI 10.1099/0022-1317-76-4-1047
© 1995 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Bruin, W. C. C.
Right arrow Articles by Yap, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Bruin, W. C. C.
Right arrow Articles by Yap, S. H.
Agricola
Right arrow Articles by de Bruin, W. C. C.
Right arrow Articles by Yap, S. H.

Hepatitus B virus: specific binding and internalization of small HBsAg by human hepatocytes

W. C. C. de Bruin1,*, K. Hertogs2, W. P. J. Leenders1, E. Depla2 and S. H. Yap2

1 Division of Gastro-intestinal and Liver Diseases, Academical Hospital Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands
and2 Division of Liver and Pancreatic Diseases, Academical Hospital Gasthuisberg, Leuven, Belgium

Previously, we identified human liver endonexin II (EII) present on human hepatocyte plasma membrane as a specific hepatitis B surface antigen (HBsAg) binding protein. We also showed the spontaneous development of anti-idiotypic (anti-HBsAg) antibodies in rabbits immunized with EII and in chicken immunized with the F(ab')2 fragment of rabbit anti-EII IgG. These findings suggest the existence of a receptor-ligand relationship between EII and HBsAg. In the present study, we demonstrate that small HBsAg conjugated to 10 nm colloidal gold also binds specifically to human hepatocytes. Invagination of the coated pit region at the HBsAg binding sites on the human hepatocyte plasma membrane results in the internalization of the HBsAg-gold particles. The binding and consequently the internalization of HBsAg is inhibited by anti-EII or anti-idiotypic (anti-HBsAg) antibodies. These findings indicate that EII is directly involved in the binding and uptake of hepatitis B envelope proteins.

* Author for correspondence. Fax +31 80 540103.

Received 1 September 1994; accepted 24 November 1994.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1995 by the Society for General Microbiology.