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1 Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden
2 Department of Clinical and Tumorimmunology, Daniel den Hoed Center, Rotterdam, The Netherlands
and3 Department of Cancer Studies, CRC Laboratories, University of Birmingham, UK
The six Epstein—Barr virus (EBV) nuclear antigen proteins (EBNA-1–6) show characteristic size variations between different virus isolates; this is a feature that has been used to identify the source of virus isolates in epidemiological studies (Ebnotyping). We have now studied the correlation between restriction fragment length polymorphisms (RFLPs) within exons coding for the EBNAs and the molecular masses of the respective proteins. The B95-8 EBV strain was used as the prototype virus. The variation in apparent molecular mass of EBNA-1, -3 and -6 correlated positively with the size of RFLP coding for repeat sequences in these polypeptides. For EBNA-2, no correlation between apparent molecular mass and length of the repetitive sequences was found. The EBNA-4 protein showed virtually no variation in apparent molecular mass and RFLP size across the repeat sequence. Based on the strong correlation between apparent molecular mass and RFLP size for EBNA-6, we developed an EBNA-6 PCR assay that discriminated between different isolates of EBV. This assay offers the advantage of EBV characterization using uncultured material (e.g. throat washings, blood or biopsies), thus avoiding the selection against poorly transforming strains that occurs during establishment of lymphoblastoid cell lines required for Ebnotyping at the protein level.
* Author for correspondence. Fax +46 8 319470. e-mail kerstin.falk@mtc.ki.se
Received 31 August 1994;
accepted 21 November 1994.
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