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Morphology and antigenicity of recombinant B19 parvovirus capsids expressed in transfected COS-7 cells

J. Mori^1,

K. E. Brown^1,

J. St Amand^2,

¹ PHLS Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
and² Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada

COS-7 cells transfected with parvovirus B19-simian virus 40 (SV40) hybrid vectors have previously been shown to express B19 structural proteins. In this study the morphology and antigenicity of B19 proteins expressed in these cells were investigated. At 84 h after transfection, approximately 10% of the COS-7 cells expressed B19 antigen, and the yield was equivalent to 2 x 10³ to 2 x 10⁵ B19 particles/transfected cell. The B19 proteins self-assembled into capsids that were morphologically and antigenically similar to native B19 virions, and could substitute for native antigen in a B19 IgM assay. Recombinant capsids lacking the recently described 11 kDa protein also resembled native virions.

^* Author for correspondence. Fax +44 181 200 7874.

Present address: Clinical Research Centre, Harrow HA1 3UJ, UK.

Present address: Clinical Hematology, NIH Bethesda, Md., USA.

Present address: Neuropharmacology, Scripps Research Institute, La Jolla, Calif., USA.

Received 5 October 1994; accepted 13 December 1994.

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