| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institut de Virologie de la Faculté de Médecine, INSERM U74, Université Louis Pasteur, 3 rue Koeberlé, 67000 Strasbourg, France
Valproic acid (VPA), a simple branched-chain fatty acid having anticonvulsant activity and used in the treatment of many forms of epilepsy, markedly stimulated human cytomegalovirus (HCMV) replication in human fibroblasts (MRC-5 cells). The maximum level of stimulation was reached when cells were treated for 24 h before infection. The enhancement of virus replication correlated with an increase in the number of immediate early (IE) and early (E) antigen-positive cells. VPA also induced expression of IE antigens after transfection of fibroblasts with a plasmid containing the entire IE1–2 region. Moreover, VPA stimulated the HCMV IE1–2 promoter/enhancer-mediated expression of 
-galactosidase in a stably transfected Jurkat T cell line. Recently, VPA was shown to inhibit glutathione reductase in human red blood cells, but an action through the glutathione metabolic pathway can be eliminated in this case, since VPA decreased the intracellular level of glutathione in Jurkat T cells but not in MRC-5 cells. The ability of VPA to stimulate HCMV replication provides an attractive model for studying the molecular mechanism of the regulation of HCMV IE1–2 gene expression.
* Author for correspondence. Fax +33 88 56 63 03.
Received 19 October 1994;
accepted 31 January 1995.
This article has been cited by other articles:
|
|
 |
|
  I. J. Groves, M. B. Reeves, and J. H. Sinclair Lytic infection of permissive cells with human cytomegalovirus is regulated by an intrinsic 'pre-immediate-early' repression of viral gene expression mediated by histone post-translational modification J. Gen. Virol., October 1, 2009; 90(10): 2364 - 2374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Michaelis, T. A. T. Ha, H. W. Doerr, and J. Cinatl Jr Valproic acid interferes with antiviral treatment in human cytomegalovirus-infected endothelial cells Cardiovasc Res, February 1, 2008; 77(3): 544 - 550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Saffert and R. F. Kalejta Human Cytomegalovirus Gene Expression Is Silenced by Daxx-Mediated Intrinsic Immune Defense in Model Latent Infections Established In Vitro J. Virol., September 1, 2007; 81(17): 9109 - 9120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-h. Feng and S. C. Kenney Valproic Acid Enhances the Efficacy of Chemotherapy in EBV-Positive Tumors by Increasing Lytic Viral Gene Expression. Cancer Res., September 1, 2006; 66(17): 8762 - 8769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Michaelis, T. Suhan, A. Reinisch, A. Reisenauer, C. Fleckenstein, D. Eikel, H. Gumbel, H. W. Doerr, H. Nau, and J. Cinatl Jr Increased Replication of Human Cytomegalovirus in Retinal Pigment Epithelial Cells by Valproic Acid Depends on Histone Deacetylase Inhibition Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3451 - 3457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Lagace and M. W. Nachtigal Inhibition of Histone Deacetylase Activity by Valproic Acid Blocks Adipogenesis J. Biol. Chem., April 30, 2004; 279(18): 18851 - 18860. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
