HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liu, J.-C. Articles by Maruniak, J. E. Search for Related Content PubMed PubMed Citation Articles by Liu, J.-C. Articles by Maruniak, J. E. Agricola Articles by Liu, J.-C. Articles by Maruniak, J. E.

Nucleotide sequence and transcriptional analysis of the gp41 gene of Spodoptera frugiperda nuclear polyhedrosis virus

Entomology and Nematology Department, University of Florida, PO Box 110620, Gainesville, FL 32611-0620, USA

The Spodoptera frugiperda multiple nucleocapsid nuclear polyhedrosis virus (SfMNPV) gp41 structural protein gene was located in the 1.9 kbp EcoRI-S fragment and sequenced. An open reading frame (ORF) of 999 nucleotides was detected that encoded a protein of 332 amino acids. The gp41 gene transcript was detected after 12 h post-infection (p.i.) and remained detectable at 48 h p.i. Two major mRNAs, about 1.6 and 2.8 kb in length, were determined by Northern blot analysis. Primer extension analysis demonstrated that the gp41 gene promoter region contains three transcription start sites. Two of the gp41 gene transcription start sites were located at -42 and -41 nucleotides from the ATG translation start codon within a consensus late transcription start site (TAAG) and another transcription start site was located at -140 nucleotides from the ATG translation start codon for which no consensus motif has been determined. Comparison of SfMNPV gp41 nucleotide and amino acid sequences with the gp41 genes from Autographa californica, Bombyx mori, and Helicoverpa zea NPVs showed 60% homology of nucleotide sequences and 70% similarity of amino acid sequences.

^* Author for correspondence. Fax +1 904 392 7567. e-mail MARUN@NERVM.NERDC.UFL.EDU

Received 27 September 1994; accepted 15 February 1995.

This article has been cited by other articles:

				J. L. C. Wolff, F. H. Valicente, R. Martins, J. V. d. C. Oliveira, and P. M. d. A. Zanotto Analysis of the genome of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV-19) and of the high genomic heterogeneity in group II nucleopolyhedroviruses J. Gen. Virol., May 1, 2008; 89(5): 1202 - 1211. [Abstract] [Full Text] [PDF]

				R. L. Harrison, B. Puttler, and H. J. R. Popham Genomic sequence analysis of a fast-killing isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus J. Gen. Virol., March 1, 2008; 89(3): 775 - 790. [Abstract] [Full Text] [PDF]

				S. P. Duffy, A. M. Young, B. Morin, C. J. Lucarotti, B. F. Koop, and D. B. Levin Sequence Analysis and Organization of the Neodiprion abietis Nucleopolyhedrovirus Genome J. Virol., July 15, 2006; 80(14): 6952 - 6963. [Abstract] [Full Text] [PDF]