HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Douglas, A. J. Articles by Curran, W. L. Search for Related Content PubMed PubMed Citation Articles by Douglas, A. J. Articles by Curran, W. L. Agricola Articles by Douglas, A. J. Articles by Curran, W. L.

Identification of a 24 kDa protein expressed by chicken anaemia virus

Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stoney Road, Stormont, Belfast BT4 3SD, UK

Antisera raised against oriented peptide conjugates were used to identify and partially characterize a 24 kDa protein product expressed by chicken anaemia virus (CAV). The peptides derived from the N and C termini of the protein were shown to react against the native protein, expressed within virus-infected cells, by immunofluorescence, immunoperoxidase and immunogold thin section electron microscopy techniques. The protein product was located by immunogold single labelling in intranuclear inclusions similar to those described previously for the 13 kDa CAV protein, which causes apoptosis. The 24 kDa protein was co-localized to the nuclear inclusions with the CAV 13 kDa protein by simultaneous dual labelling immunogold electron microscopy. Following isolation of the CAV proteins by nuclei isolation and SDS-PAGE, the antisera were used to probe for the protein by immunoblotting. The antisera recognized an expressed protein product of apparent molecular mass 30 kDa. An immunofluorescence time course study of CAV protein expression was carried out and the peptide antisera reacted against the protein at 12 h post-infection. Antisera against the 13 kDa protein reacted at similar times post-infection. This was in contrast to antisera raised against the 52 kDa capsid protein which is detectable by immunofluorescence only after 24 h. The 13 kDa and 24 kDa proteins thus appear to be early antigens produced by CAV during infection.

^* Author for correspondence. Fax +44 1232 761662.

Received 8 December 1994; accepted 14 March 1995.

This article has been cited by other articles:

				J. S. Wadia, M. V. Wagner, S. A. Ezhevsky, and S. F. Dowdy Apoptin/VP3 Contains a Concentration-Dependent Nuclear Localization Signal (NLS), Not a Tumorigenic Selective NLS J. Virol., June 1, 2004; 78(11): 6077 - 6078. [Full Text] [PDF]

				L. Kakkola, K. Hedman, H. Vanrobaeys, L. Hedman, and M. Soderlund-Venermo Cloning and sequencing of TT virus genotype 6 and expression of antigenic open reading frame 2 proteins J. Gen. Virol., May 1, 2002; 83(5): 979 - 990. [Abstract] [Full Text] [PDF]