HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sung, Y. K. Articles by Coutts, R. H. A. Search for Related Content PubMed PubMed Citation Articles by Sung, Y. K. Articles by Coutts, R. H. A. Agricola Articles by Sung, Y. K. Articles by Coutts, R. H. A.

Mutational analysis of potato yellow mosaic geminivirus

Biology Department, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, UK

Mutations have been inserted into the virion and complementary sense ORFs encoding proteins with M_rs in excess of 9 kDa of both DNA A and DNA B of potato yellow mosaic geminivirus (PYMV). Wild-type and mutant monomeric clones were tested for their ability to replicate, produce PYMV-specific DNA, spread and cause symptoms in Nicotiana benthamiana plants following biolistic inoculation. Dimeric clones of the DNA A mutants were also investigated by agroinoculation of leaf discs. In contrast to N. benthamiana plants agro-inoculated with PYMV DNA A, in which the wild-type DNA A component was capable of limited independent replication and spread, both excised DNA A and B components were required for DNA replication and symptom development in plants inoculated by the biolistic method. Mixtures of both genomic components were also infectious for potato plants following biolistic inoculation. Mutations in ORFs AL1, AL2, BR1 and BL1 resulted in clones incapable of infecting N. benthamiana plants. However, the AL2 mutation, but not the AL1 mutation, allowed viral DNA replication in leaf discs. Mutations to both the AR1 and AL3 ORFs produced clones which were infectious in plants but showed a considerable delay in the production of attenuated symptoms as compared to wild-type infections. Mutating the AL3 ORF dramatically reduced viral DNA replication in both whole plants and leaf discs. Mutations to the AL4 ORF produced clones which were as infectious for both N. benthamiana and potato plants as the wild-type clones. Our results are compared with those from mutagenesis studies on related bipartite geminiviruses.

^* Author for correspondence. Fax +44 171 584 2056. e-mail r.coutts@ic.ac.uk

Received 8 December 1994; accepted 4 April 1995.

This article has been cited by other articles:

				S. B. Settlage, R. G. See, and L. Hanley-Bowdoin Geminivirus C3 Protein: Replication Enhancement and Protein Interactions J. Virol., August 1, 2005; 79(15): 9885 - 9895. [Abstract] [Full Text] [PDF]