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Role of GTPase activity of murine Mx1 protein in nuclear localization and anti-influenza virus activity

Yukiyasu Asano

Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, 411, Japan

Murine Mx1 protein is an interferon-inducible GTPase which localizes in nuclei and inhibits influenza virus infection. Wild-type Mx1 and two mutant Mx1 proteins, each carrying a single mutation either in the GTP-binding motif (S50I) or in the self-assembly motif (C71S), were expressed in MDCK cells. Wild-type Mx1 localized in nuclei, forming small granules with minute dots, and inhibited influenza virus growth. Mutant S50I, which had no GTP-binding or GTPase activities, formed linear structures in nuclei and lacked anti-viral activity, while C71S appeared diffuse in nuclei as minute dots without granules, but retained the inhibitory activity against influenza virus growth. A correlation existed between GTPase activity, intranuclear distribution and antiviral activity. We concluded that GTPase activity is essential for expression of the biological activity of Mx1 protein.

^* Author for correspondence. Fax +81 559 81 6746. e-mail aishiham@lab.nig.ac.jp

On leave of absence from Rational Drug Design Laboratories, Fukushima, Fukushima 960-12, Japan.

Received 6 January 1995; accepted 7 March 1995.

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