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Expression of the tomato ringspot nepovirus movement and coat proteins in protoplasts

Fabienne Hans

Pacific Agriculture Research Centre, Agriculture and Agri-Food Canada, 6660 NW Marine Drive, Vancouver, British Columbia, V6T 1X2, Canada

Tomato ringspot nepovirus (TomRSV) produces a 45 kDa movement protein and a 58 kDa coat protein in infected plants. Accumulation of the movement protein in relation to that of the coat protein was studied in infected protoplasts using a monoclonal antibody against the movement protein and polyclonal antibodies against the coat protein. Unlike most other viral movement proteins, the TomRSV movement protein was present at late stages of infection. Pulse-chase labelling experiments revealed that the release of the movement protein from the precursor polyprotein was coordinated with that of the coat protein. However, the movement protein was less stable than the coat protein in the extractable fraction of the protoplasts. The expression pattern of the TomRSV movement protein is discussed in the light of the proposed mechanism of cell-to-cell movement of virus-like particles through tubular structures composed of the movement protein.

Present address: Instituut voor Planteziektenkundig Onderzoek (IPO-DLO), Binnenhaven 5, Postbus 9060, 6700 GW Wageningen, The Netherlands.

^* Author for correspondence. Fax +1 604 666 4994. e-mail SANFACON@PARGVA.AGR.CA

Received 21 February 1995; accepted 2 June 1995.

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