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Novel genotypes of hepatitis C virus in Thailand

¹ Virology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104, Japan
² Department of Surgery II, School of Medicine, Kanazawa University, Takara-machi 13-1, Kanazawa 920, Japan
³ Gastroenterology, Faculty of Medicine, Chaing Mai University, Chaing Mai 5000, Thailand
and⁴ Department of Pathology, Ramathibodi Hospital, Rama 6 Road, Bangkok 10400, Thailand

We examined 24 C-type hepatitis specimens from Thailand and detected hepatitis C virus (HCV) RNA in all of them by RT-nested PCR for a portion of the HCV 5' non-coding (5' NC) region and a portion of the HCV core region. However, we failed to detect HCV RNA in 11 specimens by RT-nested PCR for a portion in the non-structural protein 5 (NS5) region that has been used commonly for HCV genotyping. We designed a new primer set for a separate portion of the NS5 region. Using this primer set, we succeeded in amplifying this portion in all 24 specimens. Two novel HCV genotypes, tentatively designated HCV-VII and HCV-VIII, were identified by sequencing these amplified regions. Our newly designed primers for RT-nested PCR may be useful for diagnosing infection as well as for genotyping unidentified HCV genomes.

^* Author for correspondence. Fax +81 3 3543 2181.

Received 13 January 1995; accepted 21 April 1995.

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