| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biotech Research Institute, LG Chemical Ltd/Research Park 104-1 Munjidong, Yuseung-gu, Daejeon 305-380, Korea
The physical properties of hepatitis C virus (HCV) particles were determined by ultracentrifugation on 20–60% isopycnic sucrose density gradients. We report that (i) two populations of HCV particles were found in the sera of patients with chronic HCV infection [at high density (1.186–1.213 g/ml) and at low density (1.099–1.127 g/ml)], (ii) virus particles with high density values were associated with immunoglobulin, and (iii) virus particles with low density values accumulated base changes within a hypervariable region (HVR) of the E2 envelope domain of the RNA genome. The results indicate that base changes within the HVR of E2 lead to the accumulation of immunoglobulin-free virus particles. Therefore, these findings imply that persistent HCV infection is established as a consequence of sequence variation in the E2 envelope domain.
* Author for correspondence. Fax +82 42 862 0332. e-mail wsryu@twins.lgchem.co.kr
Received 24 February 1995;
accepted 4 May 1995.
This article has been cited by other articles:
|
|
 |
|
  S. U. Nielsen, M. F. Bassendine, A. D. Burt, C. Martin, W. Pumeechockchai, and G. L. Toms Association between Hepatitis C Virus and Very-Low-Density Lipoprotein (VLDL)/LDL Analyzed in Iodixanol Density Gradients J. Virol., March 1, 2006; 80(5): 2418 - 2428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Majeau, V. Gagne, M. Bolduc, and D. Leclerc Signal peptide peptidase promotes the formation of hepatitis C virus non-enveloped particles and is captured on the viral membrane during assembly J. Gen. Virol., November 1, 2005; 86(11): 3055 - 3064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Bartosch, G. Verney, M. Dreux, P. Donot, Y. Morice, F. Penin, J.-M. Pawlotsky, D. Lavillette, and F.-L. Cosset An Interplay between Hypervariable Region 1 of the Hepatitis C Virus E2 Glycoprotein, the Scavenger Receptor BI, and High-Density Lipoprotein Promotes both Enhancement of Infection and Protection against Neutralizing Antibodies J. Virol., July 1, 2005; 79(13): 8217 - 8229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zahn and J.-P. Allain Hepatitis C virus and hepatitis B virus bind to heparin: purification of largely IgG-free virions from infected plasma by heparin chromatography J. Gen. Virol., March 1, 2005; 86(3): 677 - 685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-y. W. Yu, B. Bartosch, P. Zhang, Z.-p. Guo, P. M. Renzi, L.-m. Shen, C. Granier, S. M. Feinstone, F.-L. Cosset, and R. H. Purcell Neutralizing antibodies to hepatitis C virus (HCV) in immune globulins derived from anti-HCV-positive plasma PNAS, May 18, 2004; 101(20): 7705 - 7710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Andre, F. Komurian-Pradel, S. Deforges, M. Perret, J. L. Berland, M. Sodoyer, S. Pol, C. Brechot, G. Paranhos-Baccala, and V. Lotteau Characterization of Low- and Very-Low-Density Hepatitis C Virus RNA-Containing Particles J. Virol., June 14, 2002; 76(14): 6919 - 6928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pietschmann, V. Lohmann, A. Kaul, N. Krieger, G. Rinck, G. Rutter, D. Strand, and R. Bartenschlager Persistent and Transient Replication of Full-Length Hepatitis C Virus Genomes in Cell Culture J. Virol., March 19, 2002; 76(8): 4008 - 4021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Maillard, K. Krawczynski, J. Nitkiewicz, C. Bronnert, M. Sidorkiewicz, P. Gounon, J. Dubuisson, G. Faure, R. Crainic, and A. Budkowska Nonenveloped Nucleocapsids of Hepatitis C Virus in the Serum of Infected Patients J. Virol., September 1, 2001; 75(17): 8240 - 8250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. R. Afonso, J. Jiang, F. Penin, C. Tareau, D. Samuel, M.-A. Petit, H. Bismuth, E. Dussaix, and C. Feray Nonrandom Distribution of Hepatitis C Virus Quasispecies in Plasma and Peripheral Blood Mononuclear Cell Subsets J. Virol., November 1, 1999; 73(11): 9213 - 9221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Aoyagi, C. Ohue, K. Iida, T. Kimura, E. Tanaka, K. Kiyosawa, and S. Yagi Development of a Simple and Highly Sensitive Enzyme Immunoassay for Hepatitis C Virus Core Antigen J. Clin. Microbiol., June 1, 1999; 37(6): 1802 - 1808. [Abstract] [Full Text]
|
|
|
|
|
|
J. W. Lee, K.-m. Kim, S.-H. Jung, K. J. Lee, E.-C. Choi, Y.-C. Sung, and C.-Y. Kang Identification of a Domain Containing B-Cell Epitopes in Hepatitis C Virus E2 Glycoprotein by Using Mouse Monoclonal Antibodies J. Virol., January 1, 1999; 73(1): 11 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lerat, S. Rumin, F. Habersetzer, F. Berby, M.-A. Trabaud, C. Trepo, and G. Inchauspe In Vivo Tropism of Hepatitis C Virus Genomic Sequences in Hematopoietic Cells: Influence of Viral Load, Viral Genotype, and Cell Phenotype Blood, May 15, 1998; 91(10): 3841 - 3849. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
