HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bosma, T. J. Articles by Starkey, W. G. Search for Related Content PubMed PubMed Citation Articles by Bosma, T. J. Articles by Starkey, W. G. Agricola Articles by Bosma, T. J. Articles by Starkey, W. G.

Nucleotide sequence analysis of a major antigenic domain of the E1 glycoprotein of 22 rubella virus isolates

Department of Virology, United Medical and Dental Schools of Guy's and St Thomas's Hospitals, St Thomas's Campus, Lambeth Palace Road, London SE1 7EH, UK

We have determined the nucleotide sequence of the region of the rubella virus genome which encodes amino acids 195–296 of the E1 glycoprotein (E1-195–296) from a panel of 22 rubella viruses obtained from Europe, USA and Asia between 1963–1995. E1-195–296 contains neutralizing and haemagglutinating determinants, and may represent a major antigenic domain. The nucleotide sequence divergence of the 22 rubella viruses compared to the Therien strain sequence ranged from 0.65–7.14%. The greatest sequence divergence occurred in two rubella viruses of Indian origin, and was more than twofold greater than that previously reported for rubella virus. The majority of nucleotide changes occurring in the 22 viruses did not effect the deduced amino acid sequence of E1-195–296. Two rubella viruses isolated from cases of reinfection in pregnancy did not exhibit nucleotide sequence variation resulting in changes in the deduced amino acid sequence of E1-195–296, suggesting that antigenic change within this region of E1 is not associated with rubella reinfection. A rubella virus isolated from a synovial fluid sample exhibited a nucleotide substitution in a putative neutralization domain contained within E1-195–296. Phylogenetic analysis was performed to study the relationship between E1-195–296 coding sequences of the 22 viruses in this report and corresponding sequences of other rubella viruses in the databank.

Received 14 March 1996; accepted 13 June 1996.

This article has been cited by other articles:

				J. M. Hubschen, M. Yermalovich, G. Semeiko, E. Samoilovich, E. Blatun, S. De Landtsheer, and C. P. Muller Co-circulation of multiple rubella virus strains in Belarus forming novel genetic groups within clade 1 J. Gen. Virol., July 1, 2007; 88(7): 1960 - 1966. [Abstract] [Full Text] [PDF]

				Y. Zhou, H. Ushijima, and T. K. Frey Genomic analysis of diverse rubella virus genotypes J. Gen. Virol., March 1, 2007; 88(3): 932 - 941. [Abstract] [Full Text] [PDF]

				S. Cooray, J. M. Best, and L. Jin Time-course induction of apoptosis by wild-type and attenuated strains of rubella virus J. Gen. Virol., May 1, 2003; 84(5): 1275 - 1279. [Abstract] [Full Text] [PDF]