| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
The development of herpes simplex virus as a vector for neuronal gene delivery is dependent upon the identification and characterization of promoter elements capable of driving long-term expressio nduring latency. The majority of RNA polymerase II (pol II) promoters studied are active during acute infection but silenced during latency. In order to investigate the potential of a murine RNA polymerase I (pol I) promoter to drive reporter gene expressio nduring lytic and latent infection, we describe the construction and characterization of two recombinant viruses; SC16 LAT neo and SC16 US5 neo. These viruses contain a pol I-encephalomyocarditis virus internal ribosome entry site (EMCV IRES)-neomycin phosphotransferase gene (neoR) cassette inserted into the non-essential major latency associated transcript (LAT) and US5 regions respectively. Pol I promoter activity could be detected in the rodent BHK cell line, but not the primate derived Vero cell line — consistent with the known species specificity of such promoters. This activity was specific to a virus containing an active pol I promoter. However, in situ hybridization analyses of latently infected cervical dorsal root ganglia failed to detect pol I mediated transcription of the reporter gene indicating that the murine pol I promoter is silenced following the establishment of latency. Insertion of the pol I-EMCV IRES-neoR cassette into the major LAT locus resulted in the production of a hybrid LAT transcript during latency which was translocated to the cytoplasm of latently infected neurones.
Received 13 May 1996;
accepted 24 June 1996.
This article has been cited by other articles:
|
|
 |
|
  M. Labetoulle, S. Maillet, S. Efstathiou, S. Dezelee, E. Frau, and F. Lafay HSV1 Latency Sites after Inoculation in the Lip: Assessment of their Localization and Connections to the Eye Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 217 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hoffmann and R. G. Webster Unidirectional RNA polymerase I-polymerase II transcription system for the generation of influenza A virus from eight plasmids J. Gen. Virol., December 1, 2000; 81(12): 2843 - 2847. [Abstract] [Full Text]
|
|
|
|
|
|
A. Abendroth, A. Simmons, S. Efstathiou, and R. A. Pereira Infection with an H2 recombinant herpes simplex virus vector results in expression of MHC class I antigens on the surfaces of human neuroblastoma cells in vitro and mouse sensory neurons in vivo J. Gen. Virol., October 1, 2000; 81(10): 2375 - 2383. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A. Palmer, R. H. Branston, C. E. Lilley, M. J. Robinson, F. Groutsi, J. Smith, D. S. Latchman, and R. S. Coffin Development and Optimization of Herpes Simplex Virus Vectors for Multiple Long-Term Gene Delivery to the Peripheral Nervous System J. Virol., June 15, 2000; 74(12): 5604 - 5618. [Abstract] [Full Text]
|
|
|
|
|
|
C. Smith, R. H. Lachmann, and S. Efstathiou Expression from the herpes simplex virus type 1 latency-associated promoter in the murine central nervous system J. Gen. Virol., March 1, 2000; 81(3): 649 - 662. [Abstract] [Full Text]
|
|
|
|
|
|
C. M. Preston Repression of viral transcription during herpes simplex virus latency J. Gen. Virol., January 1, 2000; 81(1): 1 - 19. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
