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J Gen Virol 77 (1996), 2591-2596; DOI 10.1099/0022-1317-77-10-2591
© 1996 Society for General Microbiology
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Expression of human cytomegalovirus ppUL83 (pp65) in a stable cell line and its association with metaphase chromosomes

Paola Dal Monte1, Christine Bessia2, Maria P. Landini1 and Susan Michelson2

1 Department of Clinical and Experimental Medicine, Section of Microbiology, St Orsola General Hospital, Via Massarenti 9, 40138 Bologna, Italy
2 Bologna, Italy, and Unité d'Immunologie Virale, Institut Pasteur, Paris, France

Astrocytoma cells were transfected with an expression vector for the structural phosphoprotein ppUL83 (pp65) of human cytomegalovirus (HCMV). Once made, pp65 showed the same characteristics as naturally produced protein and was stable for several days in astrocytoma cells, as it is during HCMV replication in fibroblasts. In permanently transfected cells, pp65 was homogeneously dispersed in the cell nucleus and showed a preferential methanol-stable association with condensed chromatin throughout cell division. Furthermore, pp65 could be detected bound to metaphase-arrested chromosomes in pp65-expressing astrocytoma cells, and in fibroblasts and astrocytoma cells during productive virus infection.

Received 31 January 1996; accepted 7 May 1996.


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