J Gen Virol Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 77 (1996), 2775-2780; DOI 10.1099/0022-1317-77-11-2775
© 1996 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, G. T.
Right arrow Articles by Rho, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, G. T.
Right arrow Articles by Rho, H. M.
Agricola
Right arrow Articles by Park, G. T.
Right arrow Articles by Rho, H. M.

The retinoblastoma gene product activates transcription of the hepatitis B virus pregenomic promoter through the Sp1 binding sites

Geon Tae Park, Cheol Yong Choi and Hyune Mo Rho

Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, Seoul 151-742, Korea

The hepatitis B virus (HBV) pregenomic promoter is regulated by two enhancers and cis-elements. We have studied whether the retinoblastoma susceptibility gene product (Rb) modulates the activity of the HBV pregenomic promoter. Cotransfection of the Rb expression vector, phRB, with pCENCAT (containing the pregenomic promoter region: nt 248–1874) increased transcription from the HBV pregenomic promoter in HepG2 cells. Deletion analysis of the pregenomic promoter indicated that the region between nt -96 and -66, which contains two Sp1 binding sites, is responsible for activation by Rb. Mutation of the Sp1 binding sites abolished activation of the pregenomic promoter by Rb in the heterologous and natural promoter context. Therefore, our results suggest that Rb can activate the HBV pregenomic promoter through the Sp1 binding sites.

Received 29 April 1996; accepted 30 May 1996.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Pacini, A. Quattrone, M. Denegri, C. Fiorillo, C. Nediani, S. R. y Cajal, and P. Nassi
Transcriptional Down-regulation of Poly(ADP-ribose) Polymerase Gene Expression by E1A Binding to pRb Proteins Protects Murine Keratinocytes from Radiation-induced Apoptosis
J. Biol. Chem., December 3, 1999; 274(49): 35107 - 35112.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. H. Choi, G. T. Park, and H. M. Rho
Interaction of Hepatitis B Viral X Protein and CCAAT/ Enhancer-binding Protein alpha  Synergistically Activates the Hepatitis B Viral Enhancer II/Pregenomic Promoter
J. Biol. Chem., January 29, 1999; 274(5): 2858 - 2865.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Y. Choi, B. H. Choi, G. T. Park, and H. M. Rho
Activating Transcription Factor 2 (ATF2) Down-regulates Hepatitis B Virus X Promoter Activity by the Competition for the Activating Protein 1 Binding Site and the Formation of the ATF2-Jun Heterodimer
J. Biol. Chem., July 4, 1997; 272(27): 16934 - 16939.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1996 by the Society for General Microbiology.