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J Gen Virol 77 (1996), 167-171; DOI 10.1099/0022-1317-77-2-167
© 1996 Society for General Microbiology
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Physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus

Chi-Ju Chen1, Douglas J. Leisy5 and Suzanne M. Thiem1,2,3,4,*

1 Program in Genetics
2 Department of Entomology
3 Department of Microbiology
and4 Pesticide Research Center, Michigan State University, East Lansing, MI 48824
and5 Department of Agricultural Chemistry, Oregon State University, Corvallis, OR 97331, USA

A physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus (AfMNPV) DNA was constructed for restriction endonucleases EcoRI, HindIII, PstI and XhoI. The genome size was estimated to be 130 kbp. Ordering of the restriction fragments was accomplished by cross-blot hybridization, double digestion and DNA—DNA hybridization. The polyhedrin gene and homologous repeat (hr) regions were located by hybridization to the Autographa californica MNPV (AcMNPV) polyhedrin gene and hr4, respectively. Restriction pattern comparison and Southern blot analysis suggest that AfMNPV is closely related to AcMNPV.

* Author for correspondence. Fax +1 517 353 5598. e-mail smthiem@ibm.cl.msu.edu

Received 1 August 1995; accepted 20 October 1995.


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