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Sequence conservation within the major capsid protein of human papillomavirus (HPV) type 18 and formation of HPV-18 virus-like particles in Saccharomyces cerevisiae

¹ Department of Virus and Cell Biology, Merck Research Laboratories, Sumneytown Pike, West Point, Pennsylvania 19486
² Division of Infectious Diseases, Indiana University School of Medicine and Roudebush Veterans Administration Medical Center, Indianapolis, Indiana 46202-5124
and³ Loyola University Medical Center, Maywood, Illinois 60153, USA

The major capsid protein L1 of human papillomaviruses (HPVs) has been identified as a promising candidate antigen for a prophylactic HPV vaccine. Since amino acid sequence heterogeneity has been demonstrated for the L1 genes within individual HPV types, nucleotide sequences for L1 were determined from six HPV-18 clinical isolates and the cervical carcinoma cell line SW756 and compared to the published HPV-18 prototype sequence. The sequences were almost identical between the clinical isolates and SW756 but differed markedly from the published prototype sequence. Resequencing the prototype HPV-18 revealed that these differences were due to sequencing artifacts of the prototype HPV-18 sequence archived in GenBank. Thus, the HPV-18 L1 genes seem to display a very high level of sequence conservation. The HPV-18 L1 gene derived from SW756 was expressed in Saccharomyces cerevisiae and self-assembly of the L1 protein into viruslike particles was demonstrated.

^* Author for correspondence. Fax +1 215 652 2142. e-mail Kathrin-Jansen@Merck.com

Received 22 August 1995; accepted 31 October 1995.

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