HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Harland, J. E. Articles by MacLean, A. R. Search for Related Content PubMed PubMed Citation Articles by Harland, J. E. Articles by MacLean, A. R. Agricola Articles by Harland, J. E. Articles by MacLean, A. R.

The herpes simplex virus type 2 strain HG52 RL1 gene contains a 154 bp intron as predicted from sequence analysis

¹ MRC Virology Unit
and² Division of Virology, Institute of Virology, Church Street, Glasgow G11 5JR
and³ Department of Neurology, Southern General Hospital, University of Glasgow, Glasgow, UK

The published sequence of the herpes simplex virus (HSV) type 2 strain HG52 neurovirulence gene RL1 indicated that, unlike the HSV-1 homologue, the gene contains a 154 bp intron. This intron contains six complete and one partial copy of a 19 bp repeat which encodes a stop codon; thus all three forward frames are blocked. By RT-PCR of poly(A)⁺ RNA, using primers on either side of the proposed intron, we have isolated a partial cDNA clone corresponding to the predicted spliced mRNA.

^* Author for correspondence. Fax +44 141 337 2236. e-mail MACLOIA@vir.gla.ac.uk

Received 11 October 1995; accepted 15 November 1995.

This article has been cited by other articles:

				A. Dolan, F. E. Jamieson, C. Cunningham, B. C. Barnett, and D. J. McGeoch The Genome Sequence of Herpes Simplex Virus Type 2 J. Virol., March 1, 1998; 72(3): 2010 - 2021. [Abstract] [Full Text] [PDF]