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J Gen Virol 77 (1996), 547-554; DOI 10.1099/0022-1317-77-3-547
© 1996 Society for General Microbiology
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Baculovirus replication alters hormone-regulated host development

Eun Ju Park1,{dagger}, Chih-Ming Yin2 and John P. Burand1,2,*

1 Department of Microbiology
and2 Department of Entomology, University of Massachusetts, Amherst, MA 01003, USA

The baculovirus Lymantria dispar nuclear polyhedrosis virus interferes with insect larval development by altering the host's hormonal system. The level of haemolymph ecdysteroids, the insect moulting hormone, was found to be higher in virus-infected larvae than in uninfected controls. This was consistently observed in both fourth instars and day 5-infected fifth instars. The rate of hormone synthesis was examined by in vitro incubation of the prothoracic gland. Gland activity in virus-infected larvae was higher than controls and continued until the late stages of virus infection, even during the time that controls had ceased to secrete ecdysone after moulting. During virus replication. The prothoracic gland was observed to maintain morphological and ultrastructural characteristics indicative of ecdysone biosynthetic activities. Therefore, it is likely that the insects are no longer under the control of the normal hormonal system after virus infection. It is felt that the alteration of hormone titre and the rate of ecdysone synthesis is the result of the activity of ecdysteroid UDP-glucosyl transferase (EGT), a virus-encoded enzyme which is thought to inactivate ecdysteroids by sugar conjugation.

* Author for correspondence. Fax +1 413 545 1578. e-mail burand@microbio.UMASS.edu

{dagger} Present address: Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Received 24 November 1994; accepted 7 November 1995.




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