HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hashimoto, Y. Articles by Matsumoto, T. Search for Related Content PubMed PubMed Citation Articles by Hashimoto, Y. Articles by Matsumoto, T. Agricola Articles by Hashimoto, Y. Articles by Matsumoto, T.

Physical mapping and identification of interspersed homologous sequences in the Trichoplusia ni granulosis virus genome

Tohru Hayakawa^1,

Atsuya Saimoto^1,

¹ Department of Applied Biology, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606, Japan
and² Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14850, USA

A restriction fragment library representing 89.3% of the genome of Trichoplusia ni granulosis virus (TnGV) was constructed. The library consisted of 13 of the 16 BamHI fragments, 18 of the 22 EcoRI fragments, and 6 of the 27 PstI fragments. By restriction endonuclease and Southern blot analysis of cloned or genomic viral DNA fragments, a complete physical map of TnGV was constructed for BamHI, EcoRI, PstI and XhoI. Three interspersed homologous regions (ihs1–ihs3) were identified from hybridization experiments and sequenced. Each TnGV ihs has an approximate size of 400 bp and shows homology to the other two. The orientation of ihs2 is inverted relative to ihs1 and ihs3. TnGV ihs regions do not have repetitive motifs or palindromic sequences, in contrast to homologous regions (hrs) of nuclear polyhedrosis viruses (NPVs). The genomic locations of TnGV ihs1–ihs3, represented in percentage map units, were very similar to those of ihs sequences previously reported in Bombyx mori NPV, suggesting that the ihs may be a novel type of cis-acting element common among baculoviruses. Additionally, an inverted repeat sequence, having overlapping multiple inverted repeats of 400 bp, was identified to the left of ihs3 on the linearized genome map of TnGV.

^* Author for correspondence. Fax +81 75 724 7760/7710. e-mail lmp@ipc.kit.ac.jp

Present address: Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan.

Present address: Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co. Ltd, Tokyo 115, Japan.

Received 27 June 1995; accepted 20 October 1995.

This article has been cited by other articles:

				S. Mukawa and C. Goto In vivo characterization of two granuloviruses in larvae of Mythimna separata (Lepidoptera: Noctuidae) J. Gen. Virol., April 1, 2008; 89(4): 915 - 921. [Abstract] [Full Text] [PDF]

				A. Sciocco-Cap, A. D. Parola, A. V. Goldberg, P. D. Ghiringhelli, and V. Romanowski Characterization of a Granulovirus Isolated from Epinotia aporema Wals. (Lepidoptera: Tortricidae) Larvae Appl. Envir. Microbiol., August 1, 2001; 67(8): 3702 - 3706. [Abstract] [Full Text] [PDF]