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Cell-mediated immunity to pseudorabies virus: cytolytic effector cells with characteristics of lymphokine-activated killer cells lyse virus-infected and glycoprotein gB- and gC-transfected L14 cells

¹ Department of Porcine and Exotic Viral Diseases
and² Department of Immunology, Institute for Animal Science and Health ID-DLO, PO Box 365, 8200 AJ Lelystad, The Netherlands

We examined cytolytic cells that lyse pseudorabies virus (PRV)-infected cells in pigs. In vitro stimulation of peripheral blood mononuclear cells from PRV-immune pigs with live PRV generated cells that lysed PRV-infected immortalized B cells. Several lines of evidence indicated a major contribution of non-major histocompatibility complex (MHC)-restricted cytolytic cells, which displayed characteristics of natural killer (NK) or lymphokine-activated killer cells: cytolysis was non-MHC-restricted, depended on CD2⁺CD4^-CD8^bright- (or CD2⁺CD4^-CD8^dull+) cells, was strongly augmented by in vitro antigenic stimulation and was not limited to virus-infected cells, i.e. the NK cell-susceptible target cell line K562 was also lysed. Cytolytic cells were also generated by in vitro antigenic stimulation with UV-inactivated PRV. Target cells transfected with and stably expressing PRV gB or gC were lysed to the same degree as PRV-infected target cells.

^* Author for correspondence. Present address: Research Laboratory for Infectious Diseases, RIVM, PO Box 1, 3720 BA Bilthoven, The Netherlands. Fax +31 30 2744449. e-mail TG.Kimman@RIVM.NL

Received 10 October 1995; accepted 8 January 1996.

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