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J Gen Virol 77 (1996), 1123-1127; DOI 10.1099/0022-1317-77-6-1123
© 1996 Society for General Microbiology
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Translational stop codons in the precore sequence of hepatitis B virus pre-C RNA allow translation reinitiation at downstream AUGs

Nathalie Fouillot{dagger} and Jean-Michel Rossignol*

Laboratoire de Génétique des Virus, UPR 2431-CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette cedex, France

Hepatitis B virus (HBV) wild-type pre-C RNA directs the synthesis of the HBeAg precursor but does not serve as mRNA for translation of the adjacent downstream C gene which encodes the core protein. Using bicistronic mRNA constructs that mimick pre-C RNA, we have demonstrated that this RNA likewise does not serve as messenger for translation of the P gene, which is located downstream of the C gene. However, when the pre-C RNA contains a translational stop codon at position 2 or 28 of the pre-C sequence (as in certain HBV mutants), it no longer directs synthesis of the HBeAg precursor but instead translation is initiated at downstream C and P gene AUGs. We propose that this occurs by a translation reinitiation mechanism.

* Author for correspondence. Fax +33 1 69 82 43 08. e-mail jmrossi@cnrs_gif.fr

{dagger} Present address: Department of Genetics and Microbiology, University of Geneva Medical School, CMU, 9 avenue de Champel, 10211 Geneva 4, Switzerland.

Received 1 December 1995; accepted 8 February 1996.


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