| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Istituto di Genetica Biochimica ed Evoluzionistica, Consiglio Nazionale delle Ricerche, via Abbiategrasso 207, I-27100 Pavia
and2 Virology Unit, Istituto di Clinica delle Malattie Infettive, IRCCS Policlinico San Matteo, I-27100 Pavia, Italy
Human cytomegalovirus phosphoprotein pp65 is targeted to the cell nucleus immediately after infection. Deletion and point mutation analysis of the pp65 gene expressed in insect cells showed that two hydrophilic regions (HP1 and HP2) within the pp65 C-terminal 40% each harboured an independent nuclear localization signal (NLS); strong association to the nuclear stroma also requires the N-terminal domain. Either region, when fused to chloramphenicol acetyltransferase, localized the reporter protein to the nucleus in insect cells as well as in NIH 3T3 cells and human lung fibroblasts. In addition, HP1 was found to be the target of pp65 Ser/Thr phosphorylation in insect cells and a prokaryotically expressed HP1 was actively phosphorylated in vitro by casein kinase II, for which two site clusters map in HP1. These findings indicate that pp65 includes two NLSs, one of which has the potential to be modulated by phosphorylation.
* Author for correspondence. Fax +39 382 422286. e-mail gallina@ipvgbe.igbe.pv.cnr.it
Received 29 September 1995;
accepted 12 January 1996.
This article has been cited by other articles:
|
|
 |
|
  V. Sanchez, J. A. Mahr, N. I. Orazio, and D. H. Spector Nuclear Export of the Human Cytomegalovirus Tegument Protein pp65 Requires Cyclin-Dependent Kinase Activity and the Crm1 Exporter J. Virol., November 1, 2007; 81(21): 11730 - 11736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yue, A. Kaur, S. S. Zhou, and P. A. Barry Characterization and immunological analysis of the rhesus cytomegalovirus homologue (Rh112) of the human cytomegalovirus UL83 lower matrix phosphoprotein (pp65). J. Gen. Virol., April 1, 2006; 87(Pt 4): 777 - 787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Davido, W. F. von Zagorski, W. S. Lane, and P. A. Schaffer Phosphorylation Site Mutations Affect Herpes Simplex Virus Type 1 ICP0 Function J. Virol., January 15, 2005; 79(2): 1232 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. McGregor, F. Liu, and M. R. Schleiss Molecular, Biological, and In Vivo Characterization of the Guinea Pig Cytomegalovirus (CMV) Homologs of the Human CMV Matrix Proteins pp71 (UL82) and pp65 (UL83) J. Virol., September 15, 2004; 78(18): 9872 - 9889. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Odeberg, B. Plachter, L. Branden, and C. Soderberg-Naucler Human cytomegalovirus protein pp65 mediates accumulation of HLA-DR in lysosomes and destruction of the HLA-DR {alpha}-chain Blood, June 15, 2003; 101(12): 4870 - 4877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Sanchez, C. L. Clark, J. Y. Yen, R. Dwarakanath, and D. H. Spector Viable Human Cytomegalovirus Recombinant Virus with an Internal Deletion of the IE2 86 Gene Affects Late Stages of Viral Replication J. Virol., February 22, 2002; 76(6): 2973 - 2989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Zaia, G. Gallez-Hawkins, X. Li, Z.-Q. Yao, N. Lomeli, K. Molinder, C. La Rosa, and D. J. Diamond Infrequent Occurrence of Natural Mutations in the pp65495-503 Epitope Sequence Presented by the HLA A*0201 Allele among Human Cytomegalovirus Isolates J. Virol., March 1, 2001; 75(5): 2472 - 2474. [Abstract] [Full Text]
|
|
|
|
|
|
G. Gerna, E. Percivalle, F. Baldanti, S. Sozzani, P. Lanzarini, E. Genini, D. Lilleri, and M. G. Revello Human Cytomegalovirus Replicates Abortively in Polymorphonuclear Leukocytes after Transfer from Infected Endothelial Cells via Transient Microfusion Events J. Virol., June 15, 2000; 74(12): 5629 - 5638. [Abstract] [Full Text]
|
|
|
|
|
|
E. Beuken, G. Grauls, C. A. Bruggeman, and C. Vink The rat cytomegalovirus R32 gene encodes a virion-associated protein that elicits a strong humoral immune response in infected rats J. Gen. Virol., October 1, 1999; 80(10): 2719 - 2728. [Abstract] [Full Text]
|
|
|
|
|
|
C. S. Morello, L. D. Cranmer, and D. H. Spector In Vivo Replication, Latency, and Immunogenicity of Murine Cytomegalovirus Mutants with Deletions in the M83 and M84 Genes, the Putative Homologs of Human Cytomegalovirus pp65 (UL83) J. Virol., September 1, 1999; 73(9): 7678 - 7693. [Abstract] [Full Text]
|
|
|
|
|
|
M. C. Battista, G. Bergamini, M. C. Boccuni, F. Campanini, A. Ripalti, and M. P. Landini Expression and Characterization of a Novel Structural Protein of Human Cytomegalovirus, pUL25 J. Virol., May 1, 1999; 73(5): 3800 - 3809. [Abstract] [Full Text]
|
|
|
|
|
|
A. Gallina, L. Simoncini, S. Garbelli, E. Percivalle, G. Pedrali-Noy, K. S. Lee, R. L. Erikson, B. Plachter, G. Gerna, and G. Milanesi Polo-Like Kinase 1 as a Target for Human Cytomegalovirus pp65 Lower Matrix Protein J. Virol., February 1, 1999; 73(2): 1468 - 1478. [Abstract] [Full Text]
|
|
|
|
|
|
V. Sanchez, P. C. Angeletti, J. A. Engler, and W. J. Britt Localization of Human Cytomegalovirus Structural Proteins to the Nuclear Matrix of Infected Human Fibroblasts J. Virol., April 1, 1998; 72(4): 3321 - 3329. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
