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B cell-specific activation of the Epstein—Barr virus-encoded C promoter compared with the wide-range activation of the W promoter

¹ Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm
and² Department of Clinical Chemistry, University of Göteborg, Sahlgren's Hospital, S-41345 Göteborg, Sweden

We have studied the activity of reporter plasmids, carrying the Epstein—Barr virus (EBV) nuclear antigen (EBNA) promoters Wp and Cp, in a group of somatic cell hybrids obtained by fusing EBV-positive lympho-blastoid cell lines or group II/III Burkitt's lymphoma cell lines with non-B cell lines. In B/non-B cell hybrids of this type, B cell markers are extinguished as a rule, in parallel with the inactivation of Cp or Wp and down-regulation of EBNA-2-6 expression. A Wp-carrying reporter construct was active in non-B cell lines. Only cells with a B cell phenotype could support the activity of Cp-carrying plasmids. EBNA-2 transactivated Cp only in B cells. Our data suggest that while Wp can be used for EBNA transcription in B and non-B cells, Cp activity is restricted to B cells. The inability of EBNA-2 to transactivate Cp in non-B cells indicates that other factors present in B cells might be involved in Cp transactivation.

^* Author for correspondence. Fax +46 8 33 04 98.

Received 8 September 1995; accepted 29 January 1996.

This article has been cited by other articles:

				E. M. Fuentes-Panana and P. D. Ling Characterization of the CBF2 Binding Site within the Epstein-Barr Virus Latency C Promoter and Its Role in Modulating EBNA2-Mediated Transactivation J. Virol., January 1, 1998; 72(1): 693 - 700. [Abstract] [Full Text] [PDF]