| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Veterinary and Biomedical Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583-0905
and2 Swine Research Unit, National Animal Disease Center, USDA, PO Box 70, Ames, IA 50010, USA
Pestiviruses initiate infection of susceptible cells by receptor-mediated endocytosis. Cellular plasma membrane or endosomal molecules involved in translocation of these viruses into the cytosol have not been unequivocally identified. We reported previously that a mutant cell line derived from Madin-Darby bovine kidney (MDBK) cells, termed CRIB-1, was resistant to infection with bovine viral diarrhoea virus. CRIB-1 cells were also resistant to infection with classical swine fever virus and border disease virus of sheep, suggesting that entry of these three different pestiviruses into bovine cells requires a common cell membrane function. The resistance is pestivirus-specific: CRIB-1 cells were as susceptible as the parental MDBK cells to 14 other viruses of cattle and swine belonging to unrelated families. The resistance of CRIB-1 cells to pestivirus infection involves a block in virus entry since transfection of virus RNA or virus inoculation in the presence of PEG resulted in productive infection. Furthermore, quantitative analyses of the outcome of PEG-mediated infection of CRIB-1 cells indicated that the intracellular milieu was fully permissive for pestivirus replication. Binding studies revealed that virus attachment to CRIB-1 cells was not completely abrogated. These results indicate that entry of pestiviruses into MDBK cells depends on a common plasma membrane or endosomal function, which is lacking in CRIB-1 cells.
* Author for correspondence. Fax +1 402 472 9690. e-mail rdonis@unl.edu
Present address: Departamento de Medicina Veterinaria Preventiva, Universidade Federal de Santa Maria-UFSM, Santa Maria, RS, Brazil 97119.
Received 13 November 1995;
accepted 16 February 1996.
This article has been cited by other articles:
|
|
 |
|
  T. Krey, E. Moussay, H.-J. Thiel, and T. Rumenapf Role of the Low-Density Lipoprotein Receptor in Entry of Bovine Viral Diarrhea Virus J. Virol., November 1, 2006; 80(21): 10862 - 10867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-H. Liu, Y.-L. Lin, J.-P. Wang, W. Liou, R. F. Hou, Y.-C. Wu, and C.-L. Liao From the Cover: Restriction of vaccinia virus replication by a ced-3 and ced-4-dependent pathway in Caenorhabditis elegans. PNAS, March 14, 2006; 103(11): 4174 - 4179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. V. G. Gil, I. H. Ansari, V. Vassilev, D. Liang, V. C. H. Lai, W. Zhong, Z. Hong, E. J. Dubovi, and R. O. Donis The Amino-Terminal Domain of Bovine Viral Diarrhea Virus Npro Protein Is Necessary for Alpha/Beta Interferon Antagonism J. Virol., January 15, 2006; 80(2): 900 - 911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Krey, H.-J. Thiel, and T. Rumenapf Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry J. Virol., April 1, 2005; 79(7): 4191 - 4200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Maurer, T. Krey, V. Moennig, H.-J. Thiel, and T. Rumenapf CD46 Is a Cellular Receptor for Bovine Viral Diarrhea Virus J. Virol., February 15, 2004; 78(4): 1792 - 1799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Liang, I. F. Sainz, I. H. Ansari, L. H. V. G. Gil, V. Vassilev, and R. O. Donis The envelope glycoprotein E2 is a determinant of cell culture tropism in ruminant pestiviruses J. Gen. Virol., May 1, 2003; 84(5): 1269 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Hulst, H. G. P. van Gennip, and R. J. M. Moormann Passage of Classical Swine Fever Virus in Cultured Swine Kidney Cells Selects Virus Variants That Bind to Heparan Sulfate due to a Single Amino Acid Change in Envelope Protein Erns J. Virol., October 15, 2000; 74(20): 9553 - 9561. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
