| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
Neutralization of human immunodeficiency virus type 1 by adding antibody after the virus has attached to the host cell (post-attachment neutralization: PAN) was investigated using three rat monoclonal antibodies (MAbs) directed against the outer domain of the membrane protein, gp120. Two of the MAbs are specific for the CD4-binding site region and one for the V3 loop. MAb ICR39.13g (CD4-binding site region-specific; IgG2b) effected PAN efficiently at temperatures from 4 to 35 °C. MAb ICR41.1i (V3 loop-specific; IgG2c) effected PAN only at temperatures of 24 °C and below. This suggests that its V3 epitope is masked by a change in gp120 which occurs at temperatures 
26 °C, or that the virion function which is inhibited by ICR41.1i and is responsible for neutralization has already operated at 26 °C. Resistance to neutralization by ICR41.1i occurred within 20 min of shifting the temperature up to 35 °C. Finally, MAb ICR39.3b (CD4-binding site region-specific; IgG2b) did not give PAN at any temperature, indicating that neutralization can only occur if this MAb binds virus before it attaches to the cell. Thus, these studies identify at least one novel fusion-independent event, the neutralization target of a V3 MAb, which occurs very early in the initial stages of virus-cell interaction.
Received 6 October 1995;
accepted 2 April 1996.
This article has been cited by other articles:
|
|
 |
|
  H. Haim, I. Steiner, and A. Panet Time Frames for Neutralization during the Human Immunodeficiency Virus Type 1 Entry Phase, as Monitored in Synchronously Infected Cell Cultures J. Virol., April 1, 2007; 81(7): 3525 - 3534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Heap, S. A. Reading, and N. J. Dimmock An antibody specific for the C-terminal tail of the gp41 transmembrane protein of human immunodeficiency virus type 1 mediates post-attachment neutralization, probably through inhibition of virus-cell fusion J. Gen. Virol., May 1, 2005; 86(5): 1499 - 1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Klasse and Q. J. Sattentau Occupancy and mechanism in antibody-mediated neutralization of animal viruses J. Gen. Virol., September 1, 2002; 83(9): 2091 - 2108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Edwards and N. J. Dimmock Hemagglutinin 1-Specific Immunoglobulin G and Fab Molecules Mediate Postattachment Neutralization of Influenza A Virus by Inhibition of an Early Fusion Event J. Virol., November 1, 2001; 75(21): 10208 - 10218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Spenlehauer, A. Kirn, A.-M. Aubertin, and C. Moog Antibody-Mediated Neutralization of Primary Human Immunodeficiency Virus Type 1 Isolates: Investigation of the Mechanism of Inhibition J. Virol., March 1, 2001; 75(5): 2235 - 2245. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Cleveland, T. D. Jones, and N. J. Dimmock Properties of a neutralizing antibody that recognizes a conformational form of epitope ERDRD in the gp41 C-terminal tail of human immunodeficiency virus type 1 J. Gen. Virol., May 1, 2000; 81(5): 1251 - 1260. [Abstract] [Full Text]
|
|
|
|
|
|
N. Jackson, M Levi, B Wahren, and N. Dimmock Properties and mechanism of action of a 17 amino acid, V3 loop-specific microantibody that binds to and neutralizes human immunodeficiency virus type 1 virions J. Gen. Virol., January 1, 1999; 80(1): 225 - 236. [Abstract]
|
|
|
|
|
|
P. N. Nyambi, M. K. Gorny, L. Bastiani, G. van der Groen, C. Williams, and S. Zolla-Pazner Mapping of Epitopes Exposed on Intact Human Immunodeficiency Virus Type 1 (HIV-1) Virions: a New Strategy for Studying the Immunologic Relatedness of HIV-1 J. Virol., November 1, 1998; 72(11): 9384 - 9391. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
