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Location, DNA sequence and transcriptional analysis of the DNA polymerase gene of orf virus

Anthony J. Robinson^1,

¹ Health Research Council Virus Research Unit, and Centre for Gene Research, University of Otago, PO Box 56, Dunedin, New Zealand
² Institut de Bacteriologie
and³ INSERM U74 et Institut de Virologie de la Faculté de Médicine de Strasbourg, Strasbourg, France

Degenerate oligonucleotides representing conserved regions of various DNA polymerases hybridized to a region located 26 kb from the left end of the orf virus (OV) strain NZ-2 genome. DNA sequence analysis of this region revealed a 3024 bp open reading frame able to encode a protein with 56% amino acid identity to the DNA polymerase of vaccinia virus (VAC) and with significant homology to other DNA polymerases. Early transcripts derived from the open reading frame were detected in RNA purified from OV-infected cells, and 5' ends were mapped to a region 8–19 nt downstream from an A/T-rich sequence that resembles VAC early promoters. Unlike the VAC gene, the OV DNA polymerase makes almost exclusive use of G/C coding options. Attempts to substitute the activity of the OV DNA polymerase for its VAC counterpart were unsuccessful. This may indicate that the OV DNA polymerase is incompatible with VAC accessory proteins.

Present address: CSIRO, Division of Wildlife and Ecology, PO Box 84, Lyneham, ACT, Australia.

Received 21 November 1995; accepted 19 February 1996.

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