HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by van Baalen, C. A. Articles by Osterhaus, A. D. M. E. Search for Related Content PubMed PubMed Citation Articles by van Baalen, C. A. Articles by Osterhaus, A. D. M. E. Agricola Articles by van Baalen, C. A. Articles by Osterhaus, A. D. M. E.

Fine-specificity of cytotoxic T lymphocytes which recognize conserved epitopes of the Gag protein of human immunodeficiency virus type 1

¹ Institute of Virology, Erasmus University, PO Box 1738, 3000 DR Rotterdam, The Netherlands
² Department of Clinical Viro-Immunology, The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands
³ Department of Vaccine Development and Immune Mechanisms, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands
⁴ UMR103, CNRS/Biomerieux, Lyon, France

Human immunodeficiency virus type 1 (HIV-1) Gag-specific cytotoxic T lymphocyte (CTL) responses were studied in seven seropositive long-term asymptomatic individuals (CDC A1) with stable CD4 counts for more than 8 years. Using a set of partially overlapping peptides covering the whole Gag, five 15–20-mer peptides were found to contain CTL epitopes. Further characterization of these epitopes revealed a new HLA-A25-restricted CTL epitope in p24, p24_203–212 ETINEEAAEW. This region of Gag is highly conserved in clades B and D of HIV-1. Naturally occurring amino acid sequences, containing p24₂₀₃D (consensus HIV-1 clades A, C, F, G and H) or p24₂₀₄I (HIV-2_ROD) were not recognized by CTL recognizing the index peptide. No virus variants with mutations in this sequence were found in peripheral blood mononuclear cells from the HIV-1-infected individual concerned during the 8 year observation period, indicating that the virus had not escaped from the observed CTL response.

Received 11 March 1996; accepted 17 April 1996.

This article has been cited by other articles:

				A. Jaffray, E. Shephard, J. van Harmelen, C. Williamson, A.-L. Williamson, and E. P. Rybicki Human immunodeficiency virus type 1 subtype C Gag virus-like particle boost substantially improves the immune response to a subtype C gag DNA vaccine in mice J. Gen. Virol., February 1, 2004; 85(2): 409 - 413. [Abstract] [Full Text] [PDF]

				W. Zhang, S. M. Lonning, and T. C. McGuire Gag Protein Epitopes Recognized by ELA-A-Restricted Cytotoxic T Lymphocytes from Horses with Long-Term Equine Infectious Anemia Virus Infection J. Virol., December 1, 1998; 72(12): 9612 - 9620. [Abstract] [Full Text] [PDF]