| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Pathology
and2 Department of Microbiology and Immunology, The Milton S. Hershey Medical Center, Hershey, PA 17033, USA
Several small, raised lesions from the underside of the tongue of domestic rabbits were isolated, and an extract prepared and tested for the presence of rabbit oral papillomavirus (ROPV). Two weeks after inoculation of this extract into the underside of rabbit tongues, multiple small discrete, grey-white nodules were observed that reached a maximum size of 2 mm in diameter by 5 weeks. These lesions showed typical ROPV pathology, and nuclei stained positive for papillomavirus (PV) group-specific antigen (GSA) by immunocytochemistry. Tissue fragments from rabbit tongues were incubated with a suspension of ROPV and placed subrenally into athymic mice. After 60 days, cysts were removed, sections cut for histology, and a virus stock prepared. GSA staining and in situ hybridization demonstrated that the xenografts were morphologically transformed with areas showing strong nuclear staining for viral capsid antigen and ROPV DNA. Extracts prepared from the pooled xenografts contained infectious ROPV as demonstrated by inoculation into the undersurface of tongues of non-immune New Zealand White rabbits. The results demonstrated that stocks of infectious ROPV can be prepared in the athymic mouse xenograft system for use in studies on the experimental transmission of a mucosal-targeting animal papillomavirus.
Received 26 January 1996;
accepted 3 April 1996.
This article has been cited by other articles:
|
|
 |
|
  R. Gambhira, S. Jagu, B. Karanam, P. E. Gravitt, T. D. Culp, N. D. Christensen, and R. B. S. Roden Protection of Rabbits against Challenge with Rabbit Papillomaviruses by Immunization with the N Terminus of Human Papillomavirus Type 16 Minor Capsid Antigen L2 J. Virol., November 1, 2007; 81(21): 11585 - 11592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gottschling, A. Stamatakis, I. Nindl, E. Stockfleth, A. Alonso, and I. G. Bravo Multiple Evolutionary Mechanisms Drive Papillomavirus Diversification Mol. Biol. Evol., May 1, 2007; 24(5): 1242 - 1258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Embers, L. R. Budgeon, M. Pickel, and N. D. Christensen Protective Immunity to Rabbit Oral and Cutaneous Papillomaviruses by Immunization with Short Peptides of L2, the Minor Capsid Protein J. Virol., August 28, 2002; 76(19): 9798 - 9805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Harvey, N. M. Cladel, L. R. Budgeon, P. A. Welsh, J. W. Griffith, C. M. Lang, and N. D. Christensen Rabbit Genital Tissue Is Susceptible to Infection by Rabbit Oral Papillomavirus: an Animal Model for a Genital Tissue-Targeting Papillomavirus J. Virol., June 1, 1998; 72(6): 5239 - 5244. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
