HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jin, Y.-M. Articles by Michalak, T. I. Search for Related Content PubMed PubMed Citation Articles by Jin, Y.-M. Articles by Michalak, T. I. Agricola Articles by Jin, Y.-M. Articles by Michalak, T. I.

Protease-activated lymphoid cell and hepatocyte recognition site in the preS1 domain of the large woodchuck hepatitis virus envelope protein

Yi-Ming Jin^1,

¹ Molecular Virology and Hepatology Research, Division of Basic Medical Sciences
and² Division of Pathology, Faculty of Medicine, Health Sciences Centre, Memorial University of Newfoundland, St John's, Newfoundland A1B 3V6, Canada

A site capable of strictly host- and cell type-specific recognition was identified in the preS1 domain of woodchuck hepatitis virus (WHV) through the use of antipeptide antisera generated against the extreme N-terminal fragment of the large virus envelope protein. The crucial determinant of this binding site was mapped to amino acids 10–13. Although a synthetic analogue of the site was highly immunogenic, natural WHV envelope did not display the site activity unless it was modified by proteolysis or acidic pH treatment, indicating an internal location of the determinant in viral envelope. Synthetic peptides encompassing the sequence of this site bound woodchuck lymphoid cells and hepatocytes in a species-restricted manner which followed characteristics of a specific ligand-receptor interaction, although their ability to interact with lymphoid cells was considerably greater than that for hepatocytes. In WHV-infected animals, a netural antibody to the identified cryptic cell-binding site arose independently of that directed against epitopes of unmodified virus envelope and its appearance constituted the earliest immunovirological indicator of virus invasion. Our results demonstrated that the preS1 domain of the large WHV envelope protein is endowed with the species- and cell type-specific recognition site which is protected against antibody surveillance by the natural tertiary structure of the protein and we suggest that proteolytic cleavage is required to induce the binding activity.

Present address: Albert Einstein College of Medicine, Bronx, New York, USA.

Received 19 December 1995; accepted 3 April 1996.

This article has been cited by other articles:

				P. M. Mulrooney-Cousins and T. I. Michalak Repeated Passage of Wild-Type Woodchuck Hepatitis Virus in Lymphoid Cells Does Not Generate Cell Type-Specific Variants or Alter Virus Infectivity J. Virol., August 1, 2008; 82(15): 7540 - 7550. [Abstract] [Full Text] [PDF]

				T. I. Michalak, P. M. Mulrooney, and C. S. Coffin Low Doses of Hepadnavirus Induce Infection of the Lymphatic System That Does Not Engage the Liver J. Virol., February 15, 2004; 78(4): 1730 - 1738. [Abstract] [Full Text] [PDF]

				Y.-Y. Lew and T. I. Michalak In Vitro and In Vivo Infectivity and Pathogenicity of the Lymphoid Cell-Derived Woodchuck Hepatitis Virus J. Virol., February 15, 2001; 75(4): 1770 - 1782. [Abstract] [Full Text]

				T. I. Michalak, P. D. Hodgson, and N. D. Churchill Posttranscriptional Inhibition of Class I Major Histocompatibility Complex Presentation on Hepatocytes and Lymphoid Cells in Chronic Woodchuck Hepatitis Virus Infection J. Virol., May 15, 2000; 74(10): 4483 - 4494. [Abstract] [Full Text]